Beta Subunit Regulation of Voltage-Gated Ca Channels

电压门控 Ca 通道的 Beta 亚基调节

• 批准号: 6445915
• 负责人: CHRISTOPHER J THALER
• 金额: $ 4.42万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6445915
• 关键词: action potentials; calcium channel; calcium flux; cell line; ganglions; gene expression; neural transmission; neurons; neuroregulation; postdoctoral investigator; protein structure; protein structure function; synapses; tissue /cell culture; transfection; voltage /patch clamp; voltage gated channel

DESCRIPTION (provided by applicant): The importance of calcium as the trigger for exocytosis has been well established making modulation of the kinetics of voltage-gated Ca channels one method through which neurons can exert regulatory control over synaptic efficacy. Ca channel beta-subunits have a strong influence over the activation, inactivation and steady-state inactivation of these channels and therefore could potentially serve as a mechanism through which alteration of Ca channel kinetics could be accomplished. This proposal is focused on testing the hypothesis that beta-subunits regulate the degree of calcium current depression produced by high frequency trains of action potentials by shifting the steady-state inactivation profiles of voltage-gated Ca channels. Beta-subunit specific modulation of cloned calcium channels will initially be examined in tsA2Ol cells and subsequently in superior cervical ganglion neurons to assess the basic characteristics and physiological significance of this proposed mechanism which may in part regulate synaptic plasticity.

描述(申请人提供)：钙作为诱因的重要性 因为胞吐作用已经被很好地建立起来了，通过调节 电压门控钙通道是神经元发挥调节作用的一种方法 对突触效能的控制。CA通道β亚基有很强的 对激活、失活和稳态失活的影响 这些渠道因此可能作为一种机制通过 这种钙通道动力学的改变是可以实现的。这项建议是 重点测试了β-亚基调节的程度 高频动作序列引起的钙电流抑制 通过改变电压门控的稳态失活曲线来实现电位 CA频道。克隆钙通道的β-亚基特异性调节将 最初在tsA2Ol细胞中进行检测，随后在上颈部进行检测 评估神经节细胞的基本特征和生理学 这一可能部分调节突触的拟议机制的意义 可塑性。