Beta Subunit Regulation of Voltage-Gated Ca Channels

描述（由申请人提供）：钙作为触发因素的重要性 已经很好地建立了胞吐作用的动力学调节， 电压门控钙通道是神经元发挥调节作用的一种方式， 控制突触功效。Ca通道β亚基具有很强的 对激活、失活和稳态失活的影响 这些渠道，因此可以作为一种机制， 可以实现Ca通道动力学的改变。这项建议是 集中于测试β亚基调节 高频动作串引起的钙电流抑制 通过改变电压门控的稳态失活曲线， 钙通道。克隆钙通道的β亚单位特异性调节将 最初在tsA201细胞中检查，随后在上级颈细胞中检查 神经节神经元的基本特征和生理功能 这一机制可能部分调节突触 可塑性。

DESCRIPTION (provided by applicant): The importance of calcium as the trigger for exocytosis has been well established making modulation of the kinetics of voltage-gated Ca channels one method through which neurons can exert regulatory control over synaptic efficacy. Ca channel beta-subunits have a strong influence over the activation, inactivation and steady-state inactivation of these channels and therefore could potentially serve as a mechanism through which alteration of Ca channel kinetics could be accomplished. This proposal is focused on testing the hypothesis that beta-subunits regulate the degree of calcium current depression produced by high frequency trains of action potentials by shifting the steady-state inactivation profiles of voltage-gated Ca channels. Beta-subunit specific modulation of cloned calcium channels will initially be examined in tsA2Ol cells and subsequently in superior cervical ganglion neurons to assess the basic characteristics and physiological significance of this proposed mechanism which may in part regulate synaptic plasticity.