CEREBELLAR MODULES AND EYE MOVEMENT CONTROL

小脑模块和眼球运动控制

• 批准号: 6480796
• 负责人: JOHN I SIMPSON
• 金额: $ 15.58万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-09-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6480796
• 关键词: brain stem; cerebellar Purkinje cell; cerebellum; eye movements; laboratory rabbit; neural information processing; olivary body; visual pathways; visual stimulus; visual tracking

DESCRIPTION (provided by applicant): The broad goal of the research is to better understand cerebellar function by investigating the neuronal signal processing underlying compensatory eye movements. For the most part the experimental method will be manipulation of the extracellularly recorded activity of Purkinje cells and interneurons in the cerebellar flocculus through presentation of natural visual and vestibular stimuli to the awake rabbit. The central theme is that signal processing in the flocculus entails mapping within and between neuronally synthesized reference frames (coordinate systems) that represent the three-dimensional geometry of the semicircular canals and the extraocular muscles, and that these reference frames have an anatomical counterpart in the modular (compartmental) organization of the flocculus. The primary focus is the signal content and synchrony of the climbing fiber input activity, recorded as Purkinje cell complex spikes, in each of the anatomically distinguishable modules. A second focus is the contribution of the Golgi cell class of interneurons to the spatiotemporal filtering of mossy fiber-granule cell activity in relation to the floccular modules. The first specific aim is to investigate the relations between the vestibularly induced modulation of complex spike activity and aspects of eye movement behavior (kinematics, vestibulo-ocular response gain, and vergence) Interaction between the complex spike modulations produced by vestibular and by full-field visual (optokinetic) stimuli will also be studied to determine the rules governing how these two signals are non-linearly combined In the second specific aim the contribution of the brainstem prepositus hypoglossi nuclei to the vestibularly induced complex spike modulation will be assessed using ablative and chemical lesions. Two other specific aims deal with complex spike synchrony and its role in motor behavior. Synchrony occurring during vestibular stimulation will be compared to that occurring without stimulation. In a related specific aim spike-triggered averaging of eye movements will be used to determine if complex spikes have a motoric effect distinguishable from that of simple spikes, and if vestibular stimulation enhances complex spike-triggered eye movements. The fifth specific aim addresses the function of a class of cerebellar interneurons thought to be Golgi cells. These cells will be anatomically identified with intracellular labeling and their modulation will be characterized using multi-axis vestibular and full-field visual stimulation.

描述（由申请人提供）：研究的主要目标是 通过研究神经元信号更好地了解小脑功能 处理潜在的补偿性眼球运动。的大部分 实验方法将是操纵细胞外记录的 小脑小叶中浦肯野细胞和中间神经元的活动， 向清醒的兔子呈现自然视觉和前庭刺激。的 中心主题是，在小叶中的信号处理需要映射内 以及神经合成的参考框架（坐标系）之间， 表示半规管的三维几何形状， 眼外肌，并且这些参考框架具有解剖学上的 在小叶的模块化（分室）组织中的对应物。的 主要焦点是爬升光纤输入的信号内容和同步性 活动，记录为浦肯野细胞复合体尖峰，在每个解剖 可区分的模块。第二个焦点是高尔基体细胞的贡献 一类中间神经元对苔藓纤维颗粒的时空滤波 细胞活性与絮状模块的关系。第一个具体目标是 研究前庭诱发的调制与 复杂的尖峰活动和眼球运动行为的方面（运动学， 前庭-眼反应增益和聚散度）复合物之间的相互作用 由前庭和全视野视觉（视动）产生的棘波调制 刺激也将被研究，以确定规则如何这两个 在第二个具体目标中， 脑干舌下前置核团对前庭诱发电位的影响 将使用消融和化学损伤来评估复合尖峰调制。 另外两个具体目标涉及复杂的尖峰同步及其在运动中的作用 行为前庭刺激期间发生的同步性将与 这是在没有刺激的情况下发生的。在一个相关的特定目标尖峰触发 眼睛运动的平均将用于确定复杂尖峰是否具有 与简单棘波的运动效应不同，如果前庭 刺激增强了复杂的尖峰触发的眼睛运动。第五具体 目的是解决一类小脑中间神经元的功能， 高尔基体细胞。这些细胞将在解剖学上与细胞内 标记和它们的调制将使用多轴前庭表征。 和全视野视觉刺激。