CEREBELLAR MODULES AND EYE MOVEMENT CONTROL

描述(由申请人提供)：研究的总体目标是 通过研究神经元信号更好地了解小脑功能 处理潜在的补偿性眼球运动。在大多数情况下， 实验方法是将手法进行细胞外记录 小脑小叶内浦肯野细胞和中间神经元的活动 将自然的视觉和前庭刺激呈现给清醒的兔子。这个 中心主题是小叶中的信号处理需要在 以及在神经元合成的参照系(坐标系)之间 表示半规管的三维几何形状和 眼外肌，这些参照系有一个解剖学上的 在小叶的模块(隔室)组织中的对应物。这个 主要关注的是上升光纤输入的信号内容和同步性 活动，记录为浦肯野细胞复合体棘波，在解剖上的每一个 可区分的模块。第二个焦点是高尔基细胞的贡献。 苔藓纤维颗粒时空过滤的中间神经元类 细胞活性与絮状组件的关系。第一个具体目标是 探讨前庭诱发的调制与脑电活动的关系。 复杂的棘波活动和眼动行为的各个方面(运动学， 前庭-眼反应增益和收敛)复合体之间的相互作用 前庭和全视野视觉(视动力)产生的棘波调制 还将对刺激进行研究，以确定支配这两个因素的规则 信号在第二个特定目标贡献中进行非线性组合 脑干舌下前置核对前庭诱发的影响 复杂的棘波调制将使用消融和化学损伤进行评估。 另外两个具体目标涉及复杂的峰同步及其在运动中的作用 行为。前庭刺激期间发生的同步性将与 在没有刺激的情况下发生。在相关的特定目标中，尖峰触发 眼球运动的平均值将被用来确定复杂的棘波是否有 运动效果区别于简单的棘波，如果是前庭的话 刺激增强了复杂的尖峰触发的眼球运动。第五个具体 目的研究一类小脑中间神经元的功能。 高尔基细胞。这些细胞将被解剖学鉴定为细胞内 标记及其调制将使用多轴前庭来表征 和全场视觉刺激。

DESCRIPTION (provided by applicant): The broad goal of the research is to better understand cerebellar function by investigating the neuronal signal processing underlying compensatory eye movements. For the most part the experimental method will be manipulation of the extracellularly recorded activity of Purkinje cells and interneurons in the cerebellar flocculus through presentation of natural visual and vestibular stimuli to the awake rabbit. The central theme is that signal processing in the flocculus entails mapping within and between neuronally synthesized reference frames (coordinate systems) that represent the three-dimensional geometry of the semicircular canals and the extraocular muscles, and that these reference frames have an anatomical counterpart in the modular (compartmental) organization of the flocculus. The primary focus is the signal content and synchrony of the climbing fiber input activity, recorded as Purkinje cell complex spikes, in each of the anatomically distinguishable modules. A second focus is the contribution of the Golgi cell class of interneurons to the spatiotemporal filtering of mossy fiber-granule cell activity in relation to the floccular modules. The first specific aim is to investigate the relations between the vestibularly induced modulation of complex spike activity and aspects of eye movement behavior (kinematics, vestibulo-ocular response gain, and vergence) Interaction between the complex spike modulations produced by vestibular and by full-field visual (optokinetic) stimuli will also be studied to determine the rules governing how these two signals are non-linearly combined In the second specific aim the contribution of the brainstem prepositus hypoglossi nuclei to the vestibularly induced complex spike modulation will be assessed using ablative and chemical lesions. Two other specific aims deal with complex spike synchrony and its role in motor behavior. Synchrony occurring during vestibular stimulation will be compared to that occurring without stimulation. In a related specific aim spike-triggered averaging of eye movements will be used to determine if complex spikes have a motoric effect distinguishable from that of simple spikes, and if vestibular stimulation enhances complex spike-triggered eye movements. The fifth specific aim addresses the function of a class of cerebellar interneurons thought to be Golgi cells. These cells will be anatomically identified with intracellular labeling and their modulation will be characterized using multi-axis vestibular and full-field visual stimulation.