CEREBELLAR MODULES AND EYE MOVEMENT CONTROL

小脑模块和眼球运动控制

• 批准号: 6642951
• 负责人: JOHN I SIMPSON
• 金额: $ 15.58万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6642951
• 关键词: brain stem; cerebellar Purkinje cell; cerebellum; eye movements; laboratory rabbit; neural information processing; olivary body; visual pathways; visual stimulus; visual tracking

DESCRIPTION (provided by applicant): The broad goal of the research is to better understand cerebellar function by investigating the neuronal signal processing underlying compensatory eye movements. For the most part the experimental method will be manipulation of the extracellularly recorded activity of Purkinje cells and interneurons in the cerebellar flocculus through presentation of natural visual and vestibular stimuli to the awake rabbit. The central theme is that signal processing in the flocculus entails mapping within and between neuronally synthesized reference frames (coordinate systems) that represent the three-dimensional geometry of the semicircular canals and the extraocular muscles, and that these reference frames have an anatomical counterpart in the modular (compartmental) organization of the flocculus. The primary focus is the signal content and synchrony of the climbing fiber input activity, recorded as Purkinje cell complex spikes, in each of the anatomically distinguishable modules. A second focus is the contribution of the Golgi cell class of interneurons to the spatiotemporal filtering of mossy fiber-granule cell activity in relation to the floccular modules. The first specific aim is to investigate the relations between the vestibularly induced modulation of complex spike activity and aspects of eye movement behavior (kinematics, vestibulo-ocular response gain, and vergence) Interaction between the complex spike modulations produced by vestibular and by full-field visual (optokinetic) stimuli will also be studied to determine the rules governing how these two signals are non-linearly combined In the second specific aim the contribution of the brainstem prepositus hypoglossi nuclei to the vestibularly induced complex spike modulation will be assessed using ablative and chemical lesions. Two other specific aims deal with complex spike synchrony and its role in motor behavior. Synchrony occurring during vestibular stimulation will be compared to that occurring without stimulation. In a related specific aim spike-triggered averaging of eye movements will be used to determine if complex spikes have a motoric effect distinguishable from that of simple spikes, and if vestibular stimulation enhances complex spike-triggered eye movements. The fifth specific aim addresses the function of a class of cerebellar interneurons thought to be Golgi cells. These cells will be anatomically identified with intracellular labeling and their modulation will be characterized using multi-axis vestibular and full-field visual stimulation.

描述（由申请人提供）：该研究的总体目标是 通过研究神经元信号更好地了解小脑功能 处理潜在的代偿性眼球运动。大部分情况下 实验方法将是操纵细胞外记录 小脑绒球中浦肯野细胞和中间神经元的活性 向清醒的兔子呈现自然的视觉和前庭刺激。这 中心主题是絮球中的信号处理需要在其中进行映射 以及神经元合成的参考系（坐标系）之间 代表半规管的三维几何形状 眼外肌，并且这些参考系具有解剖学特征 与绒球的模块化（区室）组织相对应。这 主要关注点是攀爬光纤输入的信号内容和同步性 活动，记录为浦肯野细胞复合体尖峰，在每个解剖学上 可区分的模块。第二个焦点是高尔基细胞的贡献 苔藓纤维颗粒时空过滤的中间神经元类别 与絮凝模块相关的细胞活动。第一个具体目标是 研究前庭诱导调节之间的关系 复杂的尖峰活动和眼球运动行为的各个方面（运动学、 前庭眼反应增益和聚散度）复合体之间的相互作用 由前庭和全视野视觉（视动）产生的尖峰调制 还将研究刺激以确定管理这两个因素的规则 信号是非线性组合的 在第二个具体目标中，贡献 脑干舌下核前位核对前庭诱导的 将使用烧蚀和化学损伤来评估复杂的尖峰调节。 另外两个具体目标涉及复杂的尖峰同步及其在运动中的作用 行为。前庭刺激期间发生的同步将与 在没有刺激的情况下发生的情况。在相关的特定目标中触发尖峰 眼球运动的平均值将用于确定复杂的尖峰是否具有 运动效应与简单尖峰的运动效应不同，如果前庭 刺激增强了复杂的尖峰触发的眼球运动。第五具体 目标涉及一类被认为是小脑中间神经元的功能 高尔基细胞。这些细胞将通过细胞内结构在解剖学上进行识别 标记及其调制将使用多轴前庭来表征 和全视野视觉刺激。