Mechanisms of survival and host(ile) takeovers of non-growing bacteria

非生长细菌的生存和宿主接管机制

• 批准号: 2043989
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2043989
• 关键词: Mechanisms; survival; host; ile; takeovers

Bacterial growth is often limited by availability of nutrients and most bacteria spend the majority of their time in prolonged states of very low metabolic activity and little or no growth. These non-growing states (NGS) are far less studied than other growth states. Although our knowledge of how bacteria enter the NGS has advanced in the past decade, our understanding of the factors that control metabolism in bacteria whilst in the NGS remains in its infancy. Further, bacterial viruses (phages) represent the most abundant living entities on the planet and most research on phage biology has been done in the context of growing bacteria. In Nature, however, phages largely encounter bacteria in the NGS and our knowledge of the mechanisms used by phages to successfully infect bacteria in the NGS remains elusive. The proposed PhD project will focus on mechanisms that (1) underpin how metabolism is regulated in the NGS and/or (2) allow phages to successfully infect non-growing bacteria. Since many bacteria in the NGS become refractory to diverse biotic and abiotic stresses, including antibiotics, the long-term expectation of this research is that a better understanding of how metabolism in the NGS is regulated and the strategies used by phages to infect bacteria in the NGS could inspire and inform truly novel and rational approaches to treat bacterial infections.

细菌的生长通常受到营养物质可用性的限制，并且大多数细菌大部分时间都处于代谢活性极低且很少或根本不生长的长期状态。与其他生长状态相比，对这些非生长状态 (NGS) 的研究要少得多。尽管我们对细菌如何进入 NGS 的了解在过去十年中有所进步，但我们对 NGS 中控制细菌代谢的因素的了解仍处于起步阶段。此外，细菌病毒（噬菌体）代表了地球上最丰富的生物体，大多数噬菌体生物学研究都是在细菌生长的背景下进行的。然而，在自然界中，噬菌体主要在 NGS 中遇到细菌，而我们对噬菌体成功感染 NGS 中细菌的机制的了解仍然难以捉摸。拟议的博士项目将重点关注以下机制：(1) 支持 NGS 中新陈代谢的调节方式和/或 (2) 让噬菌体成功感染非生长细菌。由于NGS中的许多细菌对包括抗生素在内的多种生物和非生物胁迫变得难以抵抗，因此这项研究的长期期望是更好地了解NGS中的代谢如何调节以及噬菌体感染NGS中的细菌所使用的策略可以激发并提供真正新颖且合理的治疗细菌感染的方法。