Mouse Model--Targeted Gene Knock-Out of Prostaglandin Sy

工作概述：一系列阐明COX基因敲除小鼠环氧化酶（COX）亚型生理功能的项目正在进行中。已经产生了COX-1和COX-2均缺乏的小鼠。这些小鼠，以及只携带一个COX基因拷贝的小鼠，被用来确定单个COX同种异构体的生理作用，并阐明这两个同种异构体单独或共同作用的方式。缺乏这两种亚型的小鼠在动脉导管未闭出生后不久死亡，但其他方面发育正常。我们发现COX-2是主要负责导管闭合的同种异构体，COX-2，而不是COX-1，在导管的收缩光滑核中被诱导

Summary of Work: A series of projects to elucidate the physiological functions of the individual cyclooxygenase (COX) isoforms are ongoing with the COX knockout mice. Mice deficient in both COX-1 and COX-2 have been produced. These mice, and mice carrying only a single copy of one of the COX genes, are being used to determine the physiological roles of the individual COX isoforms and to elucidate the ways in which the two isoforms act individually or in conjunction. Mice deficient in both isoforms die shortly after birth of patent ductus arteriosus, but otherwise appear developmentally normal. We have found that COX-2 is the isoform primarily responsible for the closure of the ductus and that COX-2, but not COX-1, is induced in the contracting smooth mucles of the ductus