The Knockout of the Mouse Muc5ac

小鼠 Muc5ac 的敲除

• 批准号: 8734152
• 负责人: Robert Langenbach
• 金额: $ 12.08万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8734152
• 关键词: Cells; Edema; Epithelial; Genes; Genomics; Goblet Cells; Human; Hyperplasia; Hypertrophy; Immunohistochemistry; Infection; Inflammation; Knock-out; Knockout Mice; Lamina Propria; Lateral; Lung; Lung Inflammation; Molecular; Mucins; Mucous body substance; Mus; Nasal cavity; Nasopharynx; Nose; Organ; PTGS2 gene; Pathway interactions; Pharmacology; Physiological; Predisposition; Reporting; Respiratory syncytial virus; Role; Stomach; Time; Wild Type Mouse; airway inflammation; conjunctiva; insight; intraepithelial; ozone exposure; research study; respiratory; response

The successful knockout of Muc5ac has been achieved and was verified by genomic Southern, PCR and Muc5ac immunohistochemistry of various organs including the gastric stomach being determined. One major observation has been that the nasopharynx and conjunctiva of the Muc5ac-/- mice have significantly fewer and smaller goblet cells which contain decreased amount of mucous. Further studies of the nasopharynx of wild type and Muc5ac-/- mice have indicated mild to moderate hypertrophy and hyperplasia of the nasal lamina propria, epithelial hyperplasia and mid-septum and lateral wall of the Muc5ac-/- mice. Following respiratory syncytial virus (RSV) administration, more extensive congestion and edema was observed throughout the nasal cavity of the Muc5ac-/- mice. Furthermore, RSV-induced intraepithelial mucus secretion was significantly lower in the -/- mice. In summary to date, the pathological responses of the -/- mice to RSV were more extreme then observed in wild type mice. These studies are continuing with RSV time course experiments and various physiological consequences being determined. In addition, the pathological consequences of ozone exposure on wild type and Muc5ac-/- mice will be compared.

Muc5ac基因成功敲除已被基因组Southern、PCR和Muc5ac免疫组化检测（包括胃）所证实。一个主要的观察结果是Muc5ac-/-小鼠的鼻咽部和结膜的杯状细胞明显减少和变小，其中含有的粘液量减少。对野生型和Muc5ac-/-小鼠鼻咽部的进一步研究表明，Muc5ac-/-小鼠的鼻固有层轻度至中度肥大、增生、上皮增生、中隔和侧壁增生。呼吸道合胞病毒（RSV）给药后，Muc5ac-/-小鼠整个鼻腔出现更广泛的充血和水肿。此外，rsv诱导的-/-小鼠上皮内粘液分泌明显降低。总之，迄今为止，-/-小鼠对RSV的病理反应比野生型小鼠更为极端。这些研究正在继续进行RSV病程实验，并确定各种生理后果。