The Knockout of the Mouse Muc5ac

小鼠 Muc5ac 的敲除

• 批准号: 8734152
• 负责人: Robert Langenbach
• 金额: $ 12.08万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8734152
• 关键词: Cells; Edema; Epithelial; Genes; Genomics; Goblet Cells; Human; Hyperplasia; Hypertrophy; Immunohistochemistry; Infection; Inflammation; Knock-out; Knockout Mice; Lamina Propria; Lateral; Lung; Lung Inflammation; Molecular; Mucins; Mucous body substance; Mus; Nasal cavity; Nasopharynx; Nose; Organ; PTGS2 gene; Pathway interactions; Pharmacology; Physiological; Predisposition; Reporting; Respiratory syncytial virus; Role; Stomach; Time; Wild Type Mouse; airway inflammation; conjunctiva; insight; intraepithelial; ozone exposure; research study; respiratory; response

The successful knockout of Muc5ac has been achieved and was verified by genomic Southern, PCR and Muc5ac immunohistochemistry of various organs including the gastric stomach being determined. One major observation has been that the nasopharynx and conjunctiva of the Muc5ac-/- mice have significantly fewer and smaller goblet cells which contain decreased amount of mucous. Further studies of the nasopharynx of wild type and Muc5ac-/- mice have indicated mild to moderate hypertrophy and hyperplasia of the nasal lamina propria, epithelial hyperplasia and mid-septum and lateral wall of the Muc5ac-/- mice. Following respiratory syncytial virus (RSV) administration, more extensive congestion and edema was observed throughout the nasal cavity of the Muc5ac-/- mice. Furthermore, RSV-induced intraepithelial mucus secretion was significantly lower in the -/- mice. In summary to date, the pathological responses of the -/- mice to RSV were more extreme then observed in wild type mice. These studies are continuing with RSV time course experiments and various physiological consequences being determined. In addition, the pathological consequences of ozone exposure on wild type and Muc5ac-/- mice will be compared.

Muc5ac的成功敲除已经实现，并通过基因组Southern、PCR和包括胃在内的多个器官的Muc5ac免疫组化进行验证。一项主要观察结果是 Muc5ac-/- 小鼠的鼻咽和结膜的杯状细胞明显更少且更小，其中含有的粘液量减少。对野生型和Muc5ac-/-小鼠鼻咽的进一步研究表明，Muc5ac-/-小鼠的鼻固有层有轻至中度的肥大和增生，上皮增生以及中隔和侧壁。施用呼吸道合胞病毒（RSV）后，在 Muc5ac-/- 小鼠的整个鼻腔中观察到更广泛的充血和水肿。此外，RSV 诱导的上皮内粘液分泌在 -/- 小鼠中显着降低。迄今为止，-/-小鼠对RSV的病理反应比在野生型小鼠中观察到的更为极端。这些研究正在继续进行，RSV 时程实验和各种生理后果正在确定。 此外，还将比较臭氧暴露对野生型和 Muc5ac-/- 小鼠的病理后果。