Cimicifuga racemosa as a serotonin modulator

升麻作为血清素调节剂

• 批准号: 6591944
• 负责人: Joanna E Burdette
• 金额: $ 3.29万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-20 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6591944
• 关键词: alternative medicine; drug discovery /isolation; estradiol; hormone regulation /control mechanism; laboratory rat; medicinal plants; menopause; neurotransmitter metabolism; receptor binding; serotonin; serotonin receptor; sign /symptom

DESCRIPTION (Provided by applicant): Despite the known correlation between estrogens and carcinogenesis, estrogen replacement therapy remains the number one treatment for menopausal symptoms. Cimicifuga racemosa is a rhizome that has been proven effective in reducing hot flashes in several clinical trials. The exact mechanism of action of the herb remains unknown. Studies may reveal that the plant works by modulating serotonin levels and thereby reduces hot flashes. If the plant ameliorates hot flashes without increasing estradiol levels, the botanical will provide a safe and effective solution for women with a history of breast cancer. The focus of this proposal is to train the applicant how to research botanicals by investigating the mechanism of Cimicifuga racemosa. The specific aims are (I.) to identify the active componant(s) of the plant using competitive serotonin receptor binding assays. (II.) Cellular in vitro studies will be conducted to determine the herbs agonistic or antagonistic properties, to establish and quantify serotonin subtype binding specificity, to characterize up- or down-regulation of receptor, and to monitor the serotonin dependent excretion of gonadotropic releasing hormones. (III.) Finally, an ovariectomized rat model will be used to evaluate the serotonergic effects of extracts and compounds in vivo.

描述（由申请人提供）：尽管已知之间的相关性 雌激素与致癌作用，雌激素替代疗法仍占多数 一种治疗更年期症状的方法。升麻是一种根茎， 多项临床试验已证明可有效减少潮热。 该草药的确切作用机制仍不清楚。研究可能揭示 该植物通过调节血清素水平来发挥作用，从而减少炎热 闪烁。如果该植物在不增加雌二醇的情况下改善潮热 水平，该植物将为女性提供安全有效的解决方案 有乳腺癌病史。该提案的重点是培训 申请人如何通过研究植物药的作用机制来研究植物药 总状升麻。具体目标是 (I.) 识别活跃的 使用竞争性血清素受体结合测定法分析植物的成分。 (II.) 将进行细胞体外研究以确定草药 激动或拮抗特性，以确定和量化血清素 亚型结合特异性，用于表征上调或下调 受体，并监测促性腺激素的血清素依赖性排泄 释放激素。 (III.)最后，将使用卵巢切除的大鼠模型 评估提取物和化合物在体内的血清素作用。