Investigation into novel immunotherapeutics which target the development of tumour associated macrophages in cancer

研究针对癌症中肿瘤相关巨噬细胞发育的新型免疫疗法

• 批准号: 2057733
• 金额: --
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2057733
• 关键词: Investigation; into; novel; immunotherapeutics; target

The proposed project will utilise spontaneous in vivo models of breast cancer and a novel transgenic model that has recently been developed by the laboratory. These models will be used alongside transcriptomic analyses, gene knockdown studies, and known drug screens, to gain biological insight into the key signals which direct the pro-tumoural phenotype of tumour associated macrophages (TAMs). Once the targets of interest have been identified they will be investigated in detail for their role in macrophage differentiation and function. Using our in vivo models, we will use intravital microscopy to track the TAMs recruitment and differentiation in real-time. The findings will be preclinically validated in human breast cancer, as well as other tumours, using in vitro macrophage culture, tumour sections and online patient survival and expression databases.

拟议的项目将利用乳腺癌的自发体内模型和实验室最近开发的新型转基因模型。这些模型将与转录组分析、基因敲低研究和已知药物筛选一起使用，以获得对指导肿瘤相关巨噬细胞（TAM）促肿瘤表型的关键信号的生物学见解。一旦确定了感兴趣的靶标，将详细研究它们在巨噬细胞分化和功能中的作用。使用我们的体内模型，我们将使用活体显微镜实时跟踪 TAM 的招募和分化。该研究结果将利用体外巨噬细胞培养、肿瘤切片以及在线患者生存和表达数据库在人类乳腺癌以及其他肿瘤中进行临床前验证。