Investigation into the Cellular Effects of Exfoliation in a Novel Human Bioengineered Skin Equivalent

研究新型人体生物工程皮肤等效物中去角质的细胞效应

• 批准号: 2748711
• 金额: --
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2748711
• 关键词: Investigation; into; Cellular; Effects; Exfoliation

Exfoliation,or removal of the skin's uppermost cells resident in the stratum corneum,is a consequence of many beauty and grooming regimes,most notably,the act of shaving.Shaving is well-known to have a detrimental impact on skin health,for example skin irritation and barrier damage. We propose,through this project, a means to model and understand skin exfoliation and mimic shaving in vitro utilising a well-characterised bioengineered skin model technology.The project will build on existing research conducted within the group,whereby the robustness of a novel in vitro bioengineered skin equivalent has been validated.Initial pilot work has demonstrated the use of tape strip technology to remove layers of the stratum corneum.The aim of this project is to further develop and characterise a methodology to study the molecular and cellular mechanisms that are stimulated by exfoliation in vitro.This project will utilise cutting edge technology in the form of human bioengineered skin developed in collaboration with P&G combined with a novel method of exfoliation to address the following objectives: 1.Optimisation & Characterisation of Tape Stripping:This student will optimise the use of tape strip technology to mimic skin barrier damage in vitro.The cellular impact of this will be measured through a range of analytical techniques,including transcriptomics,analysis of proliferation markers,epidermal thickening and transepidermal water loss (TEWL).We hypothesise that tape stripping will invoke a mild wound healing response resulting in keratinocyte hyperproliferation, epidermal thickening and impact barrier function. 2.Mechanical Insult:Alongside investigating the effect of tape stripping on the skin's barrier,this project will also aim to investigate the specific cellular effects that ensue following a physical insult to the skin.Mechanical damage can be achieved through a number of methods including: razor-damage,wounding,excoriation or scratching. This will provide valuable insight into the cellular and molecular effects of wound healing in skin,whilst providing relevant data on the biological effects of shaving to benefit the industrial sponsor.3.Application of Skincare Products & Actives:Preliminary data suggest that inflammatory responses induced by exfoliation may be attenuated by post-insult moisturisation.This project will probe this idea more deeply,investigating actives,formulations and regimes that are able to reduce some of the cellular changes that arise post-insult. 4.Application of a Neurosensory Skin Equivalent:Post-shave irritation is often perceived as a sensation induced by the exposure of neuronal cells within the skin tissue.As part of our platform skin technologies,we have developed models that include sensory neurons.The student will investigate the activation of nerve fibres following insult to the skin surface.This will provide valuable insight into the unwanted sensations that arise post-shave,but also into the fundamental biological processes that are induced post-insult.5.Application of an Immunocompetent Skin Equivalent:We hypothesise that many of the cellular changes that occur following exfoliation are the consequence of an inflammatory response.To better understand this,this project will utilize an immunocompetent version of the platform skin technology, that includes Langerhans and dendritic cells.Through exfoliation of an immunocompetent model, this project can better elucidate the cytokine cascade and cellular consequences of this inflammatory response that ensue following skin insult. This project will combine investigations into fundamental biological principles with industrial partnership. Through better understanding the cellular and molecular events that arise post-skin insult,this research will provide insight into the process of wound healing and allow for identification of points where interventions may alleviate adverse reactions.

去角质或去除居住在角质层中的皮肤的最上层细胞是许多美容和修饰方案的结果，最值得注意的是，剃须的行为。剃须的行为众所周知，众所周知对皮肤健康有不利影响，例如皮肤刺激和屏障损害。我们通过该项目提出一种方法，是一种方法来建模和理解皮肤去角质和模仿剃须的体外利用良好的生物工程性皮肤模型技术。该项目将建立在小组内进行的现有研究的基础上，从而使新型生物启动性皮肤的鲁棒性均得到了验证。 is to further develop and characterise a methodology to study the molecular and cellular mechanisms that are stimulated by exfoliation in vitro.This project will utilise cutting edge technology in the form of human bioengineered skin developed in collaboration with P&G combined with a novel method of exfoliation to address the following objectives: 1.Optimisation & Characterisation of Tape Stripping:This student will optimise the use of tape strip technology to mimic skin barrier damage in体外。将通过一系列分析技术来测量其细胞影响，包括转录组学，分析增殖标记，表皮增厚和跨表皮水损失（TEWL）。我们假设胶带剥离会在角膜脂蛋白高生产量和肉barrymeral higheral andy harbiral shigners and Expermeral功能中引起轻度的伤口愈合，并引起轻度的伤口愈合。 2.机械侮辱：除了研究胶带对皮肤屏障的影响外，该项目还将旨在调查对皮肤物理侮辱后随后发生的特定细胞效应。通过多种方法，包括：剃须刀损伤，伤害，伤害，射击，挖掘或划伤。这将为皮肤中伤口愈合的细胞和分子作用提供宝贵的见解，同时提供有关剃须以使工业赞助商受益的生物学效应的相关数据。3。皮肤护理产品和活性剂的应用：初步的数据表明，通过剥离引起的炎症反应可能会被调查后的作用。该项目的组合会更深入地进行。能够减少侵入后出现的一些细胞变化。 4.神经增感性皮肤等效的应用：剃须后刺激通常被认为是由皮肤组织中神经元细胞暴露而引起的感觉。作为我们平台皮肤技术的一部分，我们开发了感官神经元的模型，包括感觉神经元。学生将调查皮肤表面的神经纤维的激活。造成了多重的神经纤维。 。5。免疫功能相等的免疫功能相等：我们假设脱落后发生的许多细胞变化是炎症反应的结果。为了更好地理解这一点。为了更好地理解这一点，该项目将利用一种免疫性的langffffffftsone，其中包括lang effff的langientientsst.该项目可以更好地阐明皮肤侮辱后随之而来的这种炎症反应的细胞因子级联反应和细胞后果。该项目将结合对基本生物学原理的调查与工业伙伴关系。通过更好地理解皮肤后侮辱后出现的细胞和分子事件，这项研究将提供对伤口愈合过程的见解，并允许鉴定干预措施可能减轻不良反应的点。