Investigation into the Cellular Effects of Exfoliation in a Novel Human Bioengineered Skin Equivalent
研究新型人体生物工程皮肤等效物中去角质的细胞效应
基本信息
- 批准号:2748711
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:英国
- 项目类别:Studentship
- 财政年份:2022
- 资助国家:英国
- 起止时间:2022 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Exfoliation,or removal of the skin's uppermost cells resident in the stratum corneum,is a consequence of many beauty and grooming regimes,most notably,the act of shaving.Shaving is well-known to have a detrimental impact on skin health,for example skin irritation and barrier damage. We propose,through this project, a means to model and understand skin exfoliation and mimic shaving in vitro utilising a well-characterised bioengineered skin model technology.The project will build on existing research conducted within the group,whereby the robustness of a novel in vitro bioengineered skin equivalent has been validated.Initial pilot work has demonstrated the use of tape strip technology to remove layers of the stratum corneum.The aim of this project is to further develop and characterise a methodology to study the molecular and cellular mechanisms that are stimulated by exfoliation in vitro.This project will utilise cutting edge technology in the form of human bioengineered skin developed in collaboration with P&G combined with a novel method of exfoliation to address the following objectives: 1.Optimisation & Characterisation of Tape Stripping:This student will optimise the use of tape strip technology to mimic skin barrier damage in vitro.The cellular impact of this will be measured through a range of analytical techniques,including transcriptomics,analysis of proliferation markers,epidermal thickening and transepidermal water loss (TEWL).We hypothesise that tape stripping will invoke a mild wound healing response resulting in keratinocyte hyperproliferation, epidermal thickening and impact barrier function. 2.Mechanical Insult:Alongside investigating the effect of tape stripping on the skin's barrier,this project will also aim to investigate the specific cellular effects that ensue following a physical insult to the skin.Mechanical damage can be achieved through a number of methods including: razor-damage,wounding,excoriation or scratching. This will provide valuable insight into the cellular and molecular effects of wound healing in skin,whilst providing relevant data on the biological effects of shaving to benefit the industrial sponsor.3.Application of Skincare Products & Actives:Preliminary data suggest that inflammatory responses induced by exfoliation may be attenuated by post-insult moisturisation.This project will probe this idea more deeply,investigating actives,formulations and regimes that are able to reduce some of the cellular changes that arise post-insult. 4.Application of a Neurosensory Skin Equivalent:Post-shave irritation is often perceived as a sensation induced by the exposure of neuronal cells within the skin tissue.As part of our platform skin technologies,we have developed models that include sensory neurons.The student will investigate the activation of nerve fibres following insult to the skin surface.This will provide valuable insight into the unwanted sensations that arise post-shave,but also into the fundamental biological processes that are induced post-insult.5.Application of an Immunocompetent Skin Equivalent:We hypothesise that many of the cellular changes that occur following exfoliation are the consequence of an inflammatory response.To better understand this,this project will utilize an immunocompetent version of the platform skin technology, that includes Langerhans and dendritic cells.Through exfoliation of an immunocompetent model, this project can better elucidate the cytokine cascade and cellular consequences of this inflammatory response that ensue following skin insult. This project will combine investigations into fundamental biological principles with industrial partnership. Through better understanding the cellular and molecular events that arise post-skin insult,this research will provide insight into the process of wound healing and allow for identification of points where interventions may alleviate adverse reactions.
去角质,或去除皮肤角质层中最上层的细胞,是许多美容和美容制度的结果,最明显的是剃须行为。众所周知,剃须对皮肤健康有不利影响,例如皮肤刺激和屏障损伤。通过这个项目,我们提出了一种方法来模拟和理解皮肤去角质,并利用一种特性良好的生物工程皮肤模型技术模拟体外剃须。该项目将建立在小组内进行的现有研究的基础上,从而验证了一种新型体外生物工程皮肤等效物的稳健性。最初的试点工作已经证明使用胶带技术去除角质层。该项目的目的是进一步发展和表征一种方法,以研究体外剥落刺激的分子和细胞机制。该项目将利用与宝洁公司合作开发的人类生物工程皮肤形式的尖端技术,结合一种新的去角质方法,以实现以下目标:胶带剥离的优化与表征:该学生将优化胶带剥离技术的使用,以模拟体外皮肤屏障损伤。这对细胞的影响将通过一系列分析技术来测量,包括转录组学、增殖标记物分析、表皮增厚和经皮失水(TEWL)。我们假设胶带剥离会引起轻微的伤口愈合反应,导致角质细胞增生,表皮增厚和影响屏障功能。2.机械损伤:除了研究胶带剥离对皮肤屏障的影响外,该项目还将研究皮肤受到物理损伤后对细胞的特定影响。机械损伤可以通过许多方法来实现,包括:剃刀损伤、损伤、擦伤或刮伤。这将为皮肤伤口愈合的细胞和分子效应提供有价值的见解,同时提供有关剃须生物效应的相关数据,使工业赞助商受益。护肤产品和活性成分的应用:初步数据表明,损伤后保湿可以减轻由去角质引起的炎症反应。该项目将更深入地探索这一理念,研究能够减少侮辱后产生的细胞变化的活性物质、配方和机制。4.神经感觉皮肤等效物的应用:剃须后刺激通常被认为是由皮肤组织内的神经细胞暴露引起的感觉。作为我们平台皮肤技术的一部分,我们开发了包含感觉神经元的模型。学生将研究皮肤表面受到损伤后神经纤维的激活情况。这将为研究剃须后产生的不良感觉提供有价值的见解,同时也为研究受侮辱后诱发的基本生物学过程提供有价值的见解。应用具有免疫能力的皮肤等效物:我们假设在去角质后发生的许多细胞变化是炎症反应的结果。为了更好地理解这一点,该项目将利用平台皮肤技术的免疫功能版本,包括朗格汉斯和树突状细胞。通过对免疫功能模型的去角质,该项目可以更好地阐明皮肤损伤后炎症反应的细胞因子级联和细胞后果。该项目将把对基本生物学原理的研究与工业伙伴关系结合起来。通过更好地了解皮肤损伤后出现的细胞和分子事件,本研究将提供对伤口愈合过程的见解,并允许确定干预措施可能减轻不良反应的点。
项目成果
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其他文献
吉治仁志 他: "トランスジェニックマウスによるTIMP-1の線維化促進機序"最新医学. 55. 1781-1787 (2000)
Hitoshi Yoshiji 等:“转基因小鼠中 TIMP-1 的促纤维化机制”现代医学 55. 1781-1787 (2000)。
- DOI:
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LiDAR Implementations for Autonomous Vehicle Applications
- DOI:
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2021 - 期刊:
- 影响因子:0
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吉治仁志 他: "イラスト医学&サイエンスシリーズ血管の分子医学"羊土社(渋谷正史編). 125 (2000)
Hitoshi Yoshiji 等人:“血管医学与科学系列分子医学图解”Yodosha(涉谷正志编辑)125(2000)。
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Effect of manidipine hydrochloride,a calcium antagonist,on isoproterenol-induced left ventricular hypertrophy: "Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,K.,Teragaki,M.,Iwao,H.and Yoshikawa,J." Jpn Circ J. 62(1). 47-52 (1998)
钙拮抗剂盐酸马尼地平对异丙肾上腺素引起的左心室肥厚的影响:“Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,
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