MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 6464022
• 负责人: ROBERT W MAITTA
• 金额: $ 3.92万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-11-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6464022
• 关键词: SCID mouse; Streptococcus pneumoniae; Streptococcus pneumoniae vaccine; antibody titering; antigen antibody reaction; antiidiotype antibody; bacterial virus; enzyme linked immunosorbent assay; human tissue; immunocytochemistry; immunodeficiency; monoclonal antibody; passive immunization; vaccine development

The long-term objective of the study is the development of new approaches to pneumococcal vaccination that will provide better protection to patients with impaired immunity. Streptococcus pneumoniae is a pathogen responsible for great morbidity and mortality in different populations. The increase of antibiotic resistance to this and other bacteria, and the fact that current pneumococcal vaccines are not completely effective accentuate the need for better vaccines. Increased susceptibility to pneumococcal infection is particularly evident in immune impaired groups such as HIV+ individuals, newborns, bone marrow recipients, and the elderly, though S. pneumoniae causes disease in both immunocompetent and immunocompromised individuals. This proposal will use the phage-displayed technology to identify peptide mimotopes that bind to antibodies reactive with the capsular polysaccharide of serotype 8 pneumococcus (PPS-8). The peptides will be used as vaccine targets to determine if they elicit protective antibody responses in experimental pneumococcal infection. SCID-hu mice will be utilized to study the antibody responses to the peptides. Thus, human antibody responses can be studied. In addition, the reagents generated can be used to study the specificity of vaccine elicited antibodies in different patient groups.

该研究的长期目的是开发新的肺炎球菌疫苗接种方法，该方法将为免疫力受损的患者提供更好的保护。 肺炎链球菌是一种病原体，负责不同人群的发病率和死亡率。 抗生素对这种和其他细菌的耐药性增加，并且当前肺炎球菌疫苗并不完全有效地强调了对更好疫苗的需求。 在免疫受损的组（例如HIV+个体，新生儿，骨髓受体和老年人）中，对肺炎球菌感染的易感性增加尤其明显，尽管肺炎链球菌在免疫功能和免疫功能低下的个体中都会引起疾病。 该提案将使用噬菌体滴定技术来识别与抗体结合的肽模仿，该肽与血清型8肺炎球菌的囊膜多糖反应作用（PPS-8）。 肽将用作疫苗靶标，以确定它们是否在实验性肺炎球菌感染中引起保护性抗体反应。 SCID-HU小鼠将用于研究对肽的抗体反应。 因此，可以研究人类抗体反应。 另外，生成的试剂可用于研究不同患者组中疫苗的抗体的特异性。