BBSRC iCase - High-throughput genetic analysis of a butanol-producing Clostridia

BBSRC iCase - 产丁醇梭菌的高通量遗传分析

• 批准号: 2058829
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2058829
• 关键词: BBSRC; iCase; throughput; genetic; analysis

Clostridium saccharoperbutylacetonicum is a commercially important bacterial species, used for the production of the industrial solvent n-butanol. Our understanding of this species has been hampered by a lack of Clostridial genetic tools. We have developed an efficient mariner transposon delivery system which we used to construct comprehensive transposon libraries in the human pathogen C. difficile (Dembek et al, 2016). A recent BBSRC Impact Acceleration Award allowed a proof-of-principle study that demonstrated the feasibility of using this system in C. saccharoperbutylacetonicum. The aim of this project is to gain a deeper understanding of the metabolic pathways required for sporulation and growth on a variety of relevant feedstocks. This will enable rational metabolic engineering to improve growth and solvent production characteristics whilst reducing costs.

糖多丁基丙酮梭菌（Clostridium saccharoperbutylacetonicum）是一种重要的商业细菌，用于生产工业溶剂正丁醇。由于缺乏梭菌遗传工具，我们对该物种的了解受到阻碍。我们已经开发了一种有效的水手转座子传递系统，我们使用它来构建人类病原体C中的综合转座子文库。艰难梭菌（Dembek et al，2016）。最近的BBSRC冲击加速奖允许一项原理验证研究，证明了在C中使用该系统的可行性。糖多丁基丙酮该项目的目的是更深入地了解各种相关原料的孢子形成和生长所需的代谢途径。这将使合理的代谢工程，以改善生长和溶剂生产的特点，同时降低成本。