Gene Expression Arrays for Mammalian Cell Biology

描述(由申请人提供)： 我们建议在华盛顿大学为NHLBI调查人员开发一个阵列中心，该中心将利用微生物学系中现有的阵列设施--表达阵列中心(CEA)的能力。拟议的微阵列分析中心代表了华盛顿大学充满活力的研究社区与现有的高通量基因组研究能力的逻辑和协同汇合。NHLBI资助的华盛顿大学的生物学和疾病研究目前支持一支高度互动的调查队伍。拟建的中心将提供阵列和阵列服务(样品制备、杂交、载玻片扫描等)，但将专注于协助研究人员进行实验设计和数据分析。具体地说，阵列中心将： 在快速变化的技术环境中，为人类、小鼠和大鼠基因组提供最高质量的DNA阵列和阵列服务。 为研究人员提供数据分析支持和培训。 开发和提供基于统计的实验设计方法。将我们的数据分析与公共和私人表达数据库相结合。 我们建议的中心拥有强大的优势，表现在发达的微阵列分析设施、应对数据生成和数据分析挑战的专业知识以及强大的教育计划。以低成本向NHLBI研究人员提供的阵列中心的开发将允许更多的机会发现生理和疾病中基因表达的新模式，并将为基因组学应用于个体研究人员的研究计划提供重大技术进步。最后，正如华盛顿大学NHLBI资助的研究企业的代表性项目所指出的那样，我们试图应用这项技术来解决与心脏发育、血管生物学、造血、哮喘和生物材料相关的基本问题。

DESCRIPTION (provided by applicant): We propose to develop an Array Center for NHLBI investigators at the University of Washington that will leverage the capabilities of a pre-existing array facility - The Center for Expression Arrays (CEA), in the Department of Microbiology. The proposed center for microarray analysis represents a logical and synergistic confluence of a vibrant research community at the University of Washington with an existing capacity for high throughput genomic research. NHLBI funded biology and disease research at the University of Washington currently supports a highly interactive cadre of investigators. The proposed center will provide arrays and array services (sample preparation, hybridization, slide scanning, etc.), but will focus its efforts on assisting researchers with experimental design and data analysis. Specifically, the array center will: Provide access to the highest quality DNA arrays and array services for the human, mouse and rat genomes in an environment of rapidly changing technologies. Provide data analysis support and training to researchers. Develop and provide of statistically based methods of experimental design. Integrate our data analysis with public and private expression databases. Our proposed center has enormous strengths manifested in a developed facility for microarray analysis, expertise in addressing the challenges of data generation and data analysis, and a strong educational program. The development of an array center made available at low cost to NHLBI investigators will allow greater opportunities for discovery of novel patterns of gene expression in physiology and disease, and will provide a major technical advance for the application of genomics to the research programs of individual investigators. Finally, as indicated in representative projects drawn from the NHLBI funded research enterprise at the University of Washington, we seek to apply this technology to address fundamental problems relating to cardiac development, vascular biology, hematopoiesis, asthma, and biomaterials.