ASSEMBLY AND PLACEMENT OF THE CELL DIVISION RING

细胞分裂环的组装和放置

• 批准号: 6498768
• 负责人: Fred Chang
• 金额: $ 23.18万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6498768
• 关键词: Escherichia coli; Schizosaccharomyces pombe; actin binding protein; actins; cell cycle; cell cycle proteins; developmental genetics; fungal genetics; gene expression; gene interaction; green fluorescent proteins; polymerization; protein binding; protein biosynthesis; protein kinase; protein structure function; yeast two hybrid system

DESCRIPTION: This investigator has identified a novel gene, cdc12, in S. pombe that appears to play an important role in the assembly and placement of the contractile ring. He hypothesizes that during interphase, cdc12 exists in a large multiprotein complex that travels along microtubules to the middle of the cell to mark the future site of the cleavage furrow. During mitosis, the cdc12 protein is hypothesized to participate in actin ring assembly through an interaction with the actin-binding protein profilin. Dr. Chang's immediate goal is to test these hypotheses. Three specific aims are proposed: (1) to determine the function of the interaction between cdc12 and cdc3 (profilin); (2) to identify and characterize additional proteins that interact with cdc12p; and (3) to investigate the spatial aspects of ring formation by examining ring formation in living cells.

描述：本研究者在沙门氏菌中发现了一个新基因cdc12。 在组装和放置中起重要作用的粟酒 收缩环。 他假设在间期，cdc12 存在于一个大的多蛋白复合体中，该复合体沿着微管行进， 在细胞的中间标记出卵裂沟的未来位置。 在有丝分裂过程中，cdc12蛋白被假设参与肌动蛋白的合成。 通过与肌动蛋白结合蛋白的相互作用进行环组装 profilin。 张博士的近期目标是检验这些假设。 三 具体目标是：（1）确定的功能， CDC12和CDC3（profilin）之间的相互作用;（2）鉴定和 表征与CDC12p相互作用的其它蛋白质;和（3） 通过检查环来研究环形成的空间方面 在活细胞中形成。