MESOTHELIAL LUBRICATION AND PLEURAL HOMEOSTASIS

间皮润滑和胸膜稳态

• 批准号: 6499016
• 负责人: STEPHEN H LORING
• 金额: $ 23.6万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2004-07-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6499016
• 关键词: epithelium; fluid flow; histology; laboratory mouse; laboratory rabbit; laboratory rat; lymphatic circulation; mathematical model; morphometry; nonblood rheology; pleural cavity; sheep; surface property; swine

The pleural, peritoneal and pericardial cavities contain organs that must be able to change size and shape and to slide within their serous cavities to function normally. Sliding is facilitated by extremely slippery and well-lubricated mesothelial surfaces which line the cavities and cover the organs. The mesothelial cells are delicate and easily destroyed by ordinary handling. It is not known how these surfaces are lubricated or how mesothelial cells are protected from frictional damage. We propose to explore hydrodynamic mechanisms that lubricate mesothelial cells, protecting them from high shear stresses associated with sliding movements, and that help maintain an adequate layer of lubricating liquid between the sliding serosal surfaces by regulating total liquid volume. Specific Aims are to 1) measure and characterize friction between sliding mesothelial membranes; 2) observe and characterize shear-induced smoothing of serosal surfaces; 3) establish the levels of shear stress that are damaging to mesothelial surfaces; 4) measure the topography of serosal surfaces to gauge surface roughness and to define the topographical location of stomata leading to lymphatic channels; and 5) to use computational fluid dynamics and finite element analysis to evaluate hypotheses related to our findings. These studies may reveal the extent to which mesothelial surfaces of lung and chest wall come in contact, and thus may support or disprove one of two contradictory views of pleural space geometry and mechanics.

胸膜、腹膜和心包腔包含的器官必须能够改变大小和形状，并在浆液性腔内滑动才能正常运作。滑动是由极滑和润滑良好的间皮表面促进的，这些表面排列在腔内并覆盖在器官上。间皮细胞很脆弱，很容易被普通的操作破坏。目前尚不清楚这些表面是如何润滑的，也不知道间皮细胞是如何保护免受摩擦损伤的。我们建议探索润滑间皮细胞的流体力学机制，保护它们免受与滑动运动相关的高剪应力的影响，并通过调节总液量来帮助在滑动的浆膜表面之间保持足够的润滑层。具体目标是1)测量和表征滑动间皮膜之间的摩擦力；2)观察和表征剪切诱导的浆膜表面平滑；3)确定破坏间皮表面的剪应力水平；4)测量浆膜表面的形貌以测量表面粗糙度并确定通向淋巴通道的气孔的位置；以及5)使用计算流体力学和有限元分析来评估与我们的发现相关的假设。这些研究可能揭示肺和胸壁的间皮表面接触的程度，从而可能支持或反驳胸膜空间几何和力学两种相互矛盾的观点之一。