INTRODUCTORY PHASE

入门阶段

• 批准号: 6658369
• 负责人: Rachel Badovinac Ramoni
• 金额: $ 20.82万
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658369
• 关键词: INTRODUCTORY; PHASE

The purpose of this study was to determine the role of zALK-8, a novel type II serine/threonine kinase receptor, in crainofacial growth and development. To do so, DNA constructs of wild type, constitutively active, and truncated forms of this receptor were ligated into plasmid, transformed, and isolated via minipreps. 1-% agarose gels followed by sequence analysis confirmed isolation of these amplified constructs. mRNA from each construct is being employed in microinjection studies using the zebrafish as an animal model to help determine the role of this novel receptor in development. Potential ligand interactions can be predicted based on the phenotypic expression seen as the injected fish develop, however characterization and isolation of the actual ligand for this receptor is being determined through collaboration with investigators in France.

本研究的目的是确定 zALK-8（一种新型药物）的作用。 II 型丝氨酸/苏氨酸激酶受体，在颅面生长和 发展。 为此，野生型 DNA 构建体 该受体的活性形式和截短形式被连接到质粒中， 通过小量制备进行转化和分离。 1% 琼脂糖凝胶，然后 序列分析证实了这些扩增构建体的分离。 来自每个构建体的 mRNA 正在用于显微注射研究 使用斑马鱼作为动物模型来帮助确定 这种新型受体正在开发中。 潜在的配体相互作用可以 根据注射时的表型表达进行预测 鱼的发育，但是实际的特征和分离 该受体的配体正在通过与 法国的调查人员。