GENETIC EXCHANGE AND ORAL BACTERIA

遗传交换和口腔细菌

• 批准号: 6655189
• 负责人: BINGYAN WANG
• 金额: $ 17.24万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6655189
• 关键词: GENETIC; EXCHANGE; ORAL; BACTERIA

Our investigation will focus on genetic exchange among oral bacteria, especially among periodontopathic organisms. The transfer of genetic information between different organisms has been inferred from nucleotide sequence comparisons. The ecological benefits of genetic exchange include the expansion of metabolic versatility and resistance to hazardous environments. Some transformable bacteria have acquired antibiotic resistance by horizontal genetic exchange of fragments of chromosomal genes, such as penicillin-resistant penicillin-binding proteins in Streptococcus pneumoniae. The application of molecular biology techniques with oral microorganisms should permit the opportunity for an in vitro assessment of gene transfer. The specific aim of our investigation is to understand the role of genetic exchange as a risk factor in human oral infections such as periodontal diseases. Little information exists on gene transfer between the bacterial flora associated with periodontal diseases. Among the periodontopathic organisms, T. denticola, P. gingivalis, and F. nucleatum are the focus of our present investigation. The recent development of a gene transfer system in T. denticola in Dr. Kuramitsu's laboratory now makes it feasible to study whether the genetic information from T. denticola can be transferred in vitro to P. gingivalis or to F. nucleatum. We are also planning to determine if the exchange of genetic information can occur between T. denticola and oral streptococci, since the latter forms the majority of gram positive early colonizers of the tooth surface. Both in vitro bacterial culture and biofilms will be used to evaluate gene transfer from T. denticola to different bacteria. Information obtained from this study could elucidate the potential role of gene transfer between oral bacteria in the etiology of periodontal diseases. Key words: Genetic exchange, biofilms, periodontal disease

我们的研究将集中在口腔细菌之间的遗传交换， 特别是在牙周病生物体中。 转移遗传 不同生物之间的信息已经从 核苷酸序列比较。 基因的生态效益 交换包括代谢多样性和抵抗力的扩展 危险的环境。 一些可转化的细菌 抗生素耐药性的水平遗传交换片段 染色体基因，如青霉素抗性青霉素结合基因， 肺炎链球菌中的蛋白质。 分子生物学应用 口腔微生物的生物学技术应该允许 有机会在体外评估基因转移。 我们调查的具体目的是了解 遗传交换是人类口腔感染的危险因素， 牙周病 关于基因转移的信息很少 与牙周病相关的细菌植物群之间的关系。 之间 牙周病病原体T. denticola、牙龈卟啉单胞菌和F. 核质是我们目前研究的重点。 近期 建立了T.仓光医生的牙垢 实验室现在可以研究基因是否 来自T的信息齿垢菌可以在体外转移到P. gingivalis或F.具核的 我们还计划确定 T.齿垢和口腔 链球菌，因为后者形成早期革兰氏阳性的大多数 牙齿表面的殖民者。 体外细菌培养和 生物膜将用于评估T.齿垢 不同的细菌 从这项研究中获得的信息可以 阐明口腔细菌之间基因转移的潜在作用， 牙周病的病因。 关键词：基因交换，生物膜，牙周病