IDENTIFICATION OF PROTEIN CODING GENES IN HUMAN GENOMES

描述（改编自研究者摘要）：一项大规模的工作是 完成整个人类基因组的测序。识别 功能序列元件的计算工具已经变得越来越多 重要.研究人员的长期目标是使用数学和 统计学方法来识别人类基因组中的人类蛋白质编码基因。 这需要定位它们与启动子结合的大致位置， 多聚腺苷酸化信号;根据外显子描述它们的组织， 内含子和编码序列;以及通过以下方式推断它们的结构/功能： 检查它们的控制/调节元件及其编码蛋白。的 调查人员提出了以下三个更新的具体目标 应用：（1）开发数据库和计算方法， 人类和小鼠蛋白质编码基因第一外显子的鉴定。(2)到 开发用于检测近端启动子的基于进化的算法， 研究了几组特征良好的组织/发育特异性 脊椎动物基因(3)开发数据库和计算方法， 人和哺乳动物中选择性剪接外显子和转录本的鉴定 小鼠蛋白质编码基因。研究人员预测， 这个项目的成果将帮助分子生物学家找到人类基因 并且更有效和更准确地解释结构/功能。

DESCRIPTION (Adapted from the investigator's abstract): A large-scale effort is underway to complete the sequencing of the entire human genome. Identification of functional sequence elements by computational tools has become increasingly important. The investigators' long term goal is to use mathematical and statistical methods to identify human protein coding genes in the human genome. This entails locating their approximate positions bound by promoters and polyadenylation signals; delineating their organization in terms of exons, introns and coding sequences; and inferring their structure/function by examining their control/regulatory elements and their encoded proteins. The investigators propose the following three specific aims for this renewal application: (1) To develop database and computational methods for identification of first-exons in human and mouse protein-coding genes. (2) To develop an evolutionary based algorithm for detecting proximal-promoters by studying a few well-characterized sets of tissue/developmental specific vertebrate genes. (3) To develop database and computational methods for identification of alternatively spliced exons and transcripts in human and mouse protein coding genes. The investigators predict that methods and tools resulting from this project will help molecular biologists to find human genes and to interpret the structures/functions more efficiently and more accurately.