Exploiting polymer nano-encapsulation of membrane proteins for agrochemical target identification

利用膜蛋白的聚合物纳米封装进行农用化学品靶标识别

• 批准号: 2098550
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2098550
• 关键词: Exploiting; polymer; nano; encapsulation; membrane

We have previously shown that the industrial polymer, Styrene-co-maleic acid (SMA), can be used to extract membrane proteins from native membranes. This allows preparations to be made that contain pure, active and stable membrane proteins that can be used for downstream structural and functional studies. In this project we explore the use of the SMA project to make proteins of relevance to the agrochemicals sector. We will examine whether the chosen proteins can be extracted from the chosen membrane system and whether the proteins have activity. We will then explore whether modifications of the SMA structure can be used to improve the overall process.

我们之前已经证明，工业聚合物--苯乙烯-马来酸共聚物(SMA)可以用来从天然膜中提取膜蛋白。这使得可以制备含有纯净、活性和稳定的膜蛋白的制剂，这些膜蛋白可以用于下游结构和功能研究。在这个项目中，我们探索利用SMA项目来制造与农用化学品部门相关的蛋白质。我们将检查所选择的蛋白质是否可以从所选择的膜系统中提取出来，以及这些蛋白质是否具有活性。然后，我们将探讨是否可以利用修改SMA结构来改进整个进程。