Exploiting polymer nano-encapsulation of membrane proteins for agrochemical target identification

我们以前已经表明，工业聚合物，苯乙烯-共-马来酸（SMA），可用于从天然膜中提取膜蛋白。这允许制备含有纯的、活性的和稳定的膜蛋白的制剂，所述膜蛋白可用于下游结构和功能研究。在这个项目中，我们探索使用SMA项目来制造与农用化学品部门相关的蛋白质。我们将检查所选择的蛋白质是否可以从所选择的膜系统中提取，以及蛋白质是否具有活性。然后，我们将探讨是否可以使用SMA结构的修改来改善整个过程。

We have previously shown that the industrial polymer, Styrene-co-maleic acid (SMA), can be used to extract membrane proteins from native membranes. This allows preparations to be made that contain pure, active and stable membrane proteins that can be used for downstream structural and functional studies. In this project we explore the use of the SMA project to make proteins of relevance to the agrochemicals sector. We will examine whether the chosen proteins can be extracted from the chosen membrane system and whether the proteins have activity. We will then explore whether modifications of the SMA structure can be used to improve the overall process.