Innovative Technology for Gene Expression Studies

描述（由申请人提供）： Alderon Biosciences提出开发一种新的电化学方法， 特异性核酸序列的检测，而不需要酶、荧光或 放射性标记，并显示应用这种方法的可行性， 电化学板（E板）格式的基因表达研究， 癌E-Plate是传统微孔板的电子模拟物， 阅读器，将被开发作为一种工具，以确定，定量和研究mRNA， 用于癌症研究的特定目的cDNA文库。寡核苷酸 将使用含有电活性碱基的探针来捕获目标 通过在E-Plate的可单独寻址的元件上杂交来扩增序列。 探针-靶标杂交在缓冲液存在下产生信号 有一个中介。该信号可以快速读出，并用于 评估差异基因表达模式。预期的结果是 创新的电化学板和阅读器，为研究人员提供了一个 一种快速、简便且经济实惠的方法，用于探测小样本中的特定mRNA 或cDNA序列。拟议办法的另一个显著特点是， 将在人肿瘤抑制基因靶点的研究中得到证实， 能够从E板的威尔斯孔中回收捕获的靶标， 随后的克隆、测序或质谱分析。

DESCRIPTION (provided by applicant): Alderon Biosciences proposes to develop a new electrochemical method for detection of specific nucleic acid sequences without enzymatic, fluorescent or radioactive labels and to show the feasibility of applying this method in an electrochemical plate (E-Plate) format to studies of gene expression in cancer. The E-Plate, an electrical analog of a traditional microplate and reader, will be developed as a tool to identify, quantify and study mRNAs and cDNA libraries of specific interest for cancer studies. Oligonucleotide probes containing electroactive bases will be used to capture the target sequences by hybridization on separately addressable elements of the E-Plate. Probe-target hybridization generates a signal in the presence of a buffer containing a mediator. The signal can be read out rapidly and be used to evaluate differential gene expression patterns. The expected result is an innovative electrochemical plate and reader that provides researchers with a rapid, facile and affordable method to probe small samples for specific mRNA or cDNA sequences. Another distinctive feature of the proposed approach that will be demonstrated in studies with human tumor suppressor mRNA targets is the ability to recover captured targets from the wells of the E-Plate for subsequent cloning, sequencing, or mass spectrometric analysis.