Polymer Nanofluidic Field-Effect Pumping

聚合物纳米流体场效应泵送

• 批准号: 6552493
• 负责人: JUN GAO
• 金额: $ 18.73万
• 依托单位: CALIBRANT BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6552493
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; electric field; fluid flow; nanotechnology; polymers

DESCRIPTION (provided by applicant): This proposal describes a research plan for the development of a new technology for nanochannel pumping using a technique pioneered by our research team called Field-Effect Flow Control (FEFC). This technique is based on a modification of standard electronsmotic flow (EOF), with modulation of the flow velocity provided by a secondary gate voltage independent from the lengthwise EOF electric field. The technology allows for direct and independent control of flow within large numbers of interconnected nanochannels, and is expected to impact a broad range of bioanalytical instrumentation. The proposed nanofluidic FEFC pumping technology is a novel approach which will provide nanochannel flow control with specific advantages over other techniques, namely (i) independent operation of multiple pumping elements, (ii) compatibility with standard polymer nanofluidic technologies, (iii) elimination of pump leakage for 100% pump efficiency, (iv) very large scale integration of nanochannel pumping elements for complex nanofluidic systems, and (v) simple fabrication and low cost. The overall goal of this Phase I project is to demonstrate the feasibility of polymer FEFC nanopumping technology, and evaluate its relative merits compared to alternate methods of nanochannel flow control. Practical tools for the analysis of single molecules and other novel bioanalytical instrumentation are anticipated to result from advances in flow control for nanofluidic systems. One example of the potential for this technology is in the sequencing of single strands of DNA by pumping individual molecules through a nanoscale channel, with integrated electrodes interrogating the conductivity of each base (1). Other applications of great interest include nanofluidic devices to mimic biological processes, and to carry out single-molecule chemical reactions.

描述（由申请人提供）：该提案描述了一项研究计划，用于开发一种新的纳米通道泵送技术，该技术由我们的研究团队首创，称为场效应流量控制（FEFC）。这种技术是基于标准的电子运动流（electronsmotic flow）的修改，与独立于纵向的纵向电场的次级栅极电压提供的流速的调制。该技术允许直接和独立控制大量互连纳米通道内的流动，预计将影响广泛的生物分析仪器。所提出的纳米流体FEFC泵送技术是一种新颖的方法，其将提供纳米通道流动控制，其具有优于其他技术的特定优点，即（i）多个泵送元件的独立操作，（ii）与标准聚合物纳米流体技术的兼容性，（iii）消除泵泄漏以获得100%的泵效率，（iv）用于复杂纳米流体系统的纳米通道泵送元件的非常大规模的集成，以及（v）制造简单且成本低。这个第一阶段项目的总体目标是证明聚合物FEFC纳米泵技术的可行性，并评估其相对于纳米通道流动控制的替代方法的相对优点。 用于单分子分析的实用工具和其他新颖的生物分析仪器预计将从纳米流体系统的流动控制的进步中产生。该技术潜力的一个例子是通过将单个分子泵送通过纳米级通道来对DNA单链进行测序，其中集成电极询问每个碱基的导电性（1）。其他非常感兴趣的应用包括纳米流体装置，以模拟生物过程，并进行单分子化学反应。