New Labeling Reagents for Genetic Analysis

用于遗传分析的新型标记试剂

• 批准号: 6484834
• 负责人: JOHN J NALEWAY
• 金额: $ 10万
• 依托单位: MARKER GENE TECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6484834
• 关键词: cell component structure /function; fluorescent dye /probe; gene expression; genome; high performance liquid chromatography; ligands; luminescence; microarray technology; nuclear magnetic resonance spectroscopy; nucleic acid quantitation /detection; nucleic acid sequence; oligonucleotides; phosphates; reagent /indicator; technology /technique development

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project aims to investigate the feasibility of developing new, sensitive, accurate, and reliable detection methods for genomic DNA or RNA samples that are essential for genomic microarray analysis. If successful, the proposed research will help provide breakthroughs needed to advance the promising medical uses of genomic analysis to determine the pattern of gene expression in live cell assays. In Phase I of this project, Marker Gene Technologies proposes to establish the feasibility of the technology by preparing new labeling reagents and systems capable of directly modifying DNA or RNA oligomers for ultrasensitive fluorescence or chemiluminescent detection. These labeling reagents will be assayed in vitro for their ability to monitor gene expression events in response to biological effects on cell function and to monitor the change in gene expression upon application of drugs and bioactive compounds in a cell-specific manner. In Phase II, these and additional detection reagents and methods will be further tested in a variety of pre-clinical applications for genomic expression analysis combined with drug application. PROPOSED COMMERCIAL APPLICATION: The success of this project opens up enormous commercial possibilities in the fields of medical intervention in genetic diseases, genetic screening of new proteins and drugs in cell-culture systems, and new anti-bacterial and anti-viral agent discovery. In addition, it contributes new information and techniques for basic cell-biology research, and the labeling reagents and techniques produced can be marketed for these research and commercial uses.

描述（由申请人提供）：这项小型企业创新研究 第一阶段项目旨在研究开发新的、 灵敏、准确、可靠的基因组 DNA 或 RNA 检测方法 基因组微阵列分析所必需的样本。如果成功的话， 拟议的研究将有助于提供推进所需的突破 基因组分析确定基因模式的有前景的医学用途 在活细胞测定中的表达。在该项目的第一阶段，Marker Gene Technologies 提议通过以下方式确定该技术的可行性 制备能够直接修饰DNA的新型标记试剂和系统 或用于超灵敏荧光或化学发光检测的 RNA 寡聚物。 这些标记试剂将在体外检测其监测能力 基因表达事件响应对细胞功能的生物效应和 监测药物应用后基因表达的变化 以细胞特异性方式产生生物活性化合物。在第二阶段，这些和 额外的检测试剂和方法将在各种方面进一步测试 基因组表达分析结合药物的临床前应用 应用。 拟议的商业应用：该项目的成功在遗传疾病的医疗干预、细胞培养系统中新蛋白质和药物的基因筛选以及新的抗菌和抗病毒剂发现等领域开辟了巨大的商业可能性。 此外，它为基础细胞生物学研究提供了新的信息和技术，并且生产的标记试剂和技术可以销售用于这些研究和商业用途。