New Labeling Reagents for Genetic Analysis

用于遗传分析的新型标记试剂

• 批准号: 6484834
• 负责人: JOHN J NALEWAY
• 金额: $ 10万
• 依托单位: MARKER GENE TECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6484834
• 关键词: cell component structure /function; fluorescent dye /probe; gene expression; genome; high performance liquid chromatography; ligands; luminescence; microarray technology; nuclear magnetic resonance spectroscopy; nucleic acid quantitation /detection; nucleic acid sequence; oligonucleotides; phosphates; reagent /indicator; technology /technique development

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project aims to investigate the feasibility of developing new, sensitive, accurate, and reliable detection methods for genomic DNA or RNA samples that are essential for genomic microarray analysis. If successful, the proposed research will help provide breakthroughs needed to advance the promising medical uses of genomic analysis to determine the pattern of gene expression in live cell assays. In Phase I of this project, Marker Gene Technologies proposes to establish the feasibility of the technology by preparing new labeling reagents and systems capable of directly modifying DNA or RNA oligomers for ultrasensitive fluorescence or chemiluminescent detection. These labeling reagents will be assayed in vitro for their ability to monitor gene expression events in response to biological effects on cell function and to monitor the change in gene expression upon application of drugs and bioactive compounds in a cell-specific manner. In Phase II, these and additional detection reagents and methods will be further tested in a variety of pre-clinical applications for genomic expression analysis combined with drug application. PROPOSED COMMERCIAL APPLICATION: The success of this project opens up enormous commercial possibilities in the fields of medical intervention in genetic diseases, genetic screening of new proteins and drugs in cell-culture systems, and new anti-bacterial and anti-viral agent discovery. In addition, it contributes new information and techniques for basic cell-biology research, and the labeling reagents and techniques produced can be marketed for these research and commercial uses.

描述(申请者提供)：本次小型企业创新研究 第一阶段项目旨在调查开发新的、 灵敏、准确、可靠的基因组DNA或RNA检测方法 对基因组微阵列分析至关重要的样本。如果成功，则 拟议的研究将有助于提供推进 基因组分析用于确定基因模式的前景看好的医学用途 在活细胞检测中的表达。在这个项目的第一阶段，标记基因 技术公司建议通过以下方式确定该技术的可行性 制备可直接修饰DNA的新型标记试剂和体系 或用于超灵敏荧光或化学发光检测的RNA低聚物。 这些标记试剂将在体外进行检测，以了解它们的监测能力 对细胞功能和生物效应作出反应的基因表达事件 监测药物和药物应用后基因表达的变化 以细胞特有的方式合成生物活性化合物。在第二阶段，这些和 更多的检测试剂和方法将在各种情况下进一步测试 结合药物的基因组表达分析在临床前的应用 申请。 拟议的商业应用：该项目的成功为遗传疾病的医学干预、细胞培养系统中新蛋白质和药物的遗传筛选以及新的抗细菌和抗病毒药物的发现等领域开辟了巨大的商业可能性。此外，它还为基础细胞生物学研究提供了新的信息和技术，所生产的标记试剂和技术可以用于这些研究和商业用途。