New Labeling Reagents for Genetic Analysis

描述（由申请人提供）：这项小企业创新研究 第一阶段项目旨在研究开发新的， 灵敏、准确、可靠的基因组DNA或RNA检测方法 基因组微阵列分析所必需的样本。如果成功， 拟议的研究将有助于提供所需的突破， 基因组分析在医学上有很大的应用， 在活细胞测定中表达。在该项目的第一阶段，标记基因 技术建议通过以下方式确定技术的可行性： 制备能够直接修饰DNA的新标记试剂和系统 或RNA寡聚体，用于超灵敏的荧光或荧光发光检测。 将在体外测定这些标记试剂的监测能力 响应于对细胞功能的生物学效应的基因表达事件， 监测应用药物后基因表达的变化， 以细胞特异性方式产生生物活性化合物。在第二阶段， 其他检测试剂和方法将进一步在各种 基因组表达分析结合药物治疗的临床前应用 应用程序. 拟定商业应用：该项目的成功为遗传疾病的医学干预、细胞培养系统中新蛋白质和药物的遗传筛选以及新的抗菌和抗病毒剂的发现等领域开辟了巨大的商业可能性。 此外，它为基础细胞生物学研究提供了新的信息和技术，生产的标记试剂和技术可以用于这些研究和商业用途。

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project aims to investigate the feasibility of developing new, sensitive, accurate, and reliable detection methods for genomic DNA or RNA samples that are essential for genomic microarray analysis. If successful, the proposed research will help provide breakthroughs needed to advance the promising medical uses of genomic analysis to determine the pattern of gene expression in live cell assays. In Phase I of this project, Marker Gene Technologies proposes to establish the feasibility of the technology by preparing new labeling reagents and systems capable of directly modifying DNA or RNA oligomers for ultrasensitive fluorescence or chemiluminescent detection. These labeling reagents will be assayed in vitro for their ability to monitor gene expression events in response to biological effects on cell function and to monitor the change in gene expression upon application of drugs and bioactive compounds in a cell-specific manner. In Phase II, these and additional detection reagents and methods will be further tested in a variety of pre-clinical applications for genomic expression analysis combined with drug application. PROPOSED COMMERCIAL APPLICATION: The success of this project opens up enormous commercial possibilities in the fields of medical intervention in genetic diseases, genetic screening of new proteins and drugs in cell-culture systems, and new anti-bacterial and anti-viral agent discovery. In addition, it contributes new information and techniques for basic cell-biology research, and the labeling reagents and techniques produced can be marketed for these research and commercial uses.