LUCIFERASE DIRECTED SUBSTRATES FOR CELL REGULATION

用于细胞调节的荧光素酶定向底物

• 批准号: 6486189
• 负责人: JOHN J NALEWAY
• 金额: $ 9.99万
• 依托单位: MARKER GENE TECHNOLOGIES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486189
• 关键词: cell growth regulation; chemical synthesis; digital imaging; drug delivery systems; enzyme inhibitors; enzyme substrate; gene expression; genetic markers; immunoconjugates; luciferin monooxygenase; reagent /indicator; reporter genes; technology /technique development

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project aims to investigate the feasibility of developing new compounds capable of utilizing common marker gene expression in transformed cells to control the growth and character of cells in living tissue. If successful, the proposed research will provide breakthroughs needed to advance the promising medical uses of recombinant genes. In Phase I of this project, Marker Gene Technologies proposes to establish the feasibility of the technology by preparing new D-luciferin conjugates of common growth regulators, drugs and enzyme inhibitors, for administration to animal cells or bacteria that contain either the firefly luciferase gene (luc) as a gene fusion marker. These new conjugates will provide innovative methods of utilizing this reporter gene system in transformed cells to affect drug delivery and control selected biological properties. In Phase I, the new conjugates will be assayed in vitro and in vivo for their ability to cause specific and localized inhibition or improvement of cell growth and to deliver activated drugs in a cell- or tissue-specific manner, In Phase II, the conjugates will be further tested in a variety of significant medical applications.

描述（由申请人提供）：本小型企业创新研究