Aging and Endothelial Function of Muscle Arterioles
肌肉小动脉的衰老和内皮功能
基本信息
- 批准号:6509968
- 负责人:
- 金额:$ 21.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-04-01 至 2003-06-30
- 项目状态:已结题
- 来源:
- 关键词:aging animal old age arterioles enzyme inhibitors exercise gastrocnemius muscle gene expression laboratory rat mature animal messenger RNA microcirculation musculoskeletal circulation nitric oxide nitric oxide synthase polymerase chain reaction prostaglandin endoperoxide synthase prostaglandins striated muscles tissue /cell preparation vascular endothelium vascular resistance vasodilation vasodilators
项目摘要
DESCRIPTION: (Verbatim from application) Skeletal muscle perfusion and
endothelium-mediated vasodilation of the skeletal muscle resistance vasculature
appear to diminish with advancing age; however, the precise mechanisms that
underlie aging-induced decrements in vasodilatory function of the skeletal
muscle vasculature are unclear. Our recent work has documented impaired
flow-induced vasodilation in 1A arterioles from soleus and gastrocnemius
muscles of aged Fischer 344 rats. Although flow-induced vasodilation of
skeletal muscle arterioles is known to be an endothelium-dependent response,
the mechanisms of the response have not been defined in arterioles from
locomotory muscles, such as the soleus and gastrocnemius muscles, and
furthermore, the mechanisms which underlie the aging-induced impairment of this
response are not known. Therefore, we propose to investigate the contribution
of endothelium-dependent vasodilatory pathways to flow-induced vasodilation in
soleus and gastronemius muscle arterioles from young and aged Fisher 344 rats.
We will test the hypothesis that the aging-induced impairment of flow-induced
vasodilation in these resistance arterioles occurs primarily due to a decrease
in the ability of the endothelium to produce prostanoid vasodilators. We will
use specific inhibitors of key enzymes to determine which component(s) of the
flow-induced vasodilation, i.e., endothelial nitric oxide (NO), prostanoid
vasodilators, or endothelium-derived hyperpolarizing factor (EDEHF), is
downregulated in arterioles from old animals (Aim 1). We will determine whether
decreases in protein and mRNA expression for the constitutive form of
cyclooxygenase (COX-1) and endothelial nitric synthase (ecNOS) potentially
underlie reduced production of No and prostanoid vasodilators in response to
flow in arterioles from old rats (Aim2). Finally, because an increased tendency
toward more sedentary behavior occurs with advancing age and because exercise
training has been shown to improve endothelium-dependent vasodilation of the
skeletal muscle vasculature, we propose to determine whether exercise training
can ameliorate the impaired flow-induced vasodilation that occurs in skeletal
muscle arterioles from aged rats (Aim 3). Although aging has been documented to
reduce skeletal muscle vasodilatory responses, these studies will be the first
to evaluate the endothelial mechanisms responsible for the loss of vasodilatory
function. Furthermore, these studies will determine whether exercise training
can counter the aging-associated loss of endothelium-dependent vasodilatory
responses in skeletal muscle resistance arterioles.
描述:(来自应用的逐字)骨骼肌灌注和
内皮介导的骨骼肌阻力血管系统的血管扩张
似乎随着年龄的增长而减少;然而,准确的机制
增龄性骨骼血管扩张功能减退的潜在原因
肌肉血管系统尚不清楚。我们最近的工作记录了
比目鱼肌和腓肠肌的1A小动脉血流诱导的血管扩张
老年Fischer 344大鼠的肌肉。虽然血流诱导的血管扩张
众所周知,骨骼肌小动脉是一种内皮依赖性反应,
这种反应的机制还没有在小动脉中明确。
运动肌,如比目鱼肌和腓肠肌;
此外,衰老引起的脑功能损害的机制
目前尚不清楚他们的反应。因此,我们建议调查其贡献
血管内皮依赖性血管扩张通路对血流诱导的血管扩张的影响
青年和老年Fisher 344大鼠的比目鱼肌和胃肌肌小动脉。
我们将检验这样一种假设,即衰老导致的心流障碍
这些阻力小动脉的血管扩张主要是由于血管减少。
内皮细胞产生前列腺素类血管扩张剂的能力。我们会
使用关键酶的特定抑制剂来确定黄连中的哪种成分(S)
血流诱导的血管扩张,即内皮细胞一氧化氮(NO)、前列腺素
血管扩张剂,或内皮衍生超极化因子(EDEHF),是
老年动物的微动脉表达下调(目标1)。我们将确定是否
组织形式的蛋白质和mRNA表达减少
环氧合酶(COX-1)和内皮型一氧化氮合酶(EcNOS)
NO和前列腺素类血管扩张剂的产生减少是由于
老年大鼠小动脉内流量(AIM2)。最后,因为一种增加的趋势
随着年龄的增长和锻炼,会出现更多的久坐行为
训练已被证明可以改善血管内皮依赖性的血管扩张。
骨骼肌血管系统,我们建议确定是否运动训练
可以改善骨骼组织中受损的血流诱导的血管扩张
老年大鼠的肌小动脉(目标3)。尽管老龄化已被记录在案
减少骨骼肌血管扩张反应,这些研究将是第一次
评价血管舒张性丧失的内皮机制
功能。此外,这些研究将确定运动训练是否
可以对抗衰老相关的内皮依赖性血管扩张功能的丧失
骨骼肌阻力小动脉的反应。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aging impairs endothelium-dependent vasodilation in rat skeletal muscle arterioles.
衰老会损害大鼠骨骼肌小动脉的内皮依赖性血管舒张功能。
- DOI:10.1152/ajpheart.00004.2002
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Muller-Delp,JudyM;Spier,ScottA;Ramsey,MichaelW;Delp,MichaelD
- 通讯作者:Delp,MichaelD
Exercise training enhances flow-induced vasodilation in skeletal muscle resistance arteries of aged rats: role of PGI2 and nitric oxide.
运动训练增强老年大鼠骨骼肌阻力动脉中血流诱导的血管舒张:PGI2 和一氧化氮的作用。
- DOI:10.1152/ajpheart.00588.2006
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Spier,ScottA;Delp,MichaelD;Stallone,JohnN;Dominguez2nd,JamesM;Muller-Delp,JudyM
- 通讯作者:Muller-Delp,JudyM
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JUDY M DELP其他文献
JUDY M DELP的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JUDY M DELP', 18)}}的其他基金
Role of Adiponectin in Reversal of Age-related Vascular Dysfunction
脂联素在逆转年龄相关血管功能障碍中的作用
- 批准号:
10566303 - 财政年份:2023
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
6921769 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7247709 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7221294 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7391188 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7054082 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7147409 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:
Aging, Estrogen, and Coronary Endothelial Function
衰老、雌激素和冠状动脉内皮功能
- 批准号:
7743909 - 财政年份:2005
- 资助金额:
$ 21.28万 - 项目类别:














{{item.name}}会员




