Core--Microarray support

描述（由申请人提供）： 范德比尔特大学已经建立了一个最先进的微阵列设施，目前由范德比尔特-英格拉姆癌症中心，糖尿病中心和范德比尔特NIDDK生物技术中心支持。该设施为这些研究中心的成员提供高质量的微阵列生产，杂交和数据分析。核心B将使计划调查人员能够访问该资源提供的全部先进技术。项目0010、0011和0013将利用核心B进行旨在揭示与心律失常易感性相关的基因表达变化的拟议实验。在项目0010中，已经概述了在实验性心肌病的心脏中表达CaM激酶II抑制肽或对照肽的小鼠之间比较基因表达模式的实验。这些实验旨在了解CaMKII及其上游和下游调节剂对导致心力衰竭和心律失常的分子途径的作用。项目0011将利用微阵列实验研究体外细胞培养系统，用于检查快速起搏下心房肌细胞的电重构，目的是识别导致房颤易感表型的早期分子变化。项目0013开发了一种新的分子资源，从心脏中收集的犬表达序列标签（EST），将用于开发基因阵列，最初应用于与人类疾病非常相似的心律失常易感性犬模型。核心B由两个主要关键要素组成：（1）微阵列生产和杂交;以及（2）数据采集和分析。这两个元素与国家的最先进的技术和计算工具，保证上级质量和可靠性的微阵列实验。核心将支持核心运营所需的关键人员的工资、设备和其他基础设施费用，以扩大现有服务，并支持分析微阵列实验数据的专业知识。

DESCRIPTION (provided by applicant): Vanderbilt University has established a state-of-the-art microarray facility that is presently supported by the Vanderbilt-Ingram Cancer Center, the Diabetes Center and the Vanderbilt NIDDK Biotechnology Center. This facility provides high quality microarray production, hybridization and data analysis for members of these research centers. Core B will enable Program investigators access to the full spectrum of advanced technologies offered by this resource. Projects 0010, 0011 and 0013 will utilize Core B for proposed experiments aimed at revealing changes in gene expression associated with arrhythmia susceptibility. In Project 0010, experiments have been outlined to compare gene expression patterns between mice expressing a CaM Kinase II inhibitory peptide or control peptide in heart in experimental cardiomyopathies. These experiments seek knowledge of the role of CaMKII and its upstream and downstream regulators on molecular pathways leading to cardiac failure and arrhythmias. Project 0011 will utilize microarray experiments to study an in vitro cell culture system for examining electrical remodeling in atrial myocytes subjected to rapid pacing, with the goal of identifying early molecular changes contributing to an atrial fibrillation-susceptible phenotype. Project 0013 has developed a novel molecular resource, a canine expressed sequence tag (EST) collection from heart, that will be used to develop gene arrays to be applied initially to dog models of susceptibility to arrhythmias closely resembling human disease. Core B consists of two main critical elements: (1) microarray production and hybridization; and (2) data acquisition and analysis. Both elements operate with state-of-the-art technological and computational tools that guarantee superior quality and reliability in microarray experiments. The Core will support the salaries of key personnel needed for operating the core, equipment and other infrastructure costs that enable expansion of existing services, and support for expertise in analysis of the data that emerge from microarray experiments.