CORE--DNA SEQUENCING AND ANALYSIS

核心——DNA测序与分析

• 批准号: 6664430
• 负责人: HARRY A. DRABKIN
• 金额: $ 25.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6664430
• 关键词: DNA; biomedical facility; computer assisted sequence analysis; nucleic acid sequence

Description: (Applicant's Description) The DNA Sequencing and Analysis Core is now in its 4th year of highly successful operation. Initiated as a fledging operation after the inception of the CU Cancer Center, the Core has grown remarkably with now 112 user groups, of which most are Cancer Center members. In addition to performing DNA sequencing reactions, Core services include preparation of various DNA templates and assisting customers with all technical aspects of DNA sequencing and analysis. The Core also prepares high-density filter arrays using customer prepared libraries. In addition to service functions, Core personnel reported improvements in basic DNA sequencing chemistry that permit longer, more accurate read-lengths and improved mutation detection. To facilitate research efforts of the CU Hereditary Cancer Clinic, the Core now offers screening for the BRCA1/2 185delAG, 5382insC and 6174delT mutations. Especially as a consequence of the Genome Project, the demand for DNA sequencing will continue to expand significantly. During the next period of support, the Core intends to continue its state-of-the-art services to Cancer Center members. Research-based mutation/polymorphism screening is also likely to expand. Over the past 4 years samples sequenced have increased from 1501 to 9410 (526%) and charge back fees have increased from $43,095 to $187,136 (334%). Because of the large increase we have been able to reduce fees which has led to continued increases in core usage. The core has been used by members of 9 of the 10 Cancer Center programs. The core will move to the Fitzsimons campus with the Cancer Center during the next grant cycle.

描述：(申请人描述)DNA测序和分析核心 现已进入运营非常成功的第四个年头。作为初出茅庐的 运营CU癌症中心成立后，核心不断壮大 值得注意的是，现在有112个用户组，其中大多数是癌症中心的成员。 除了执行DNA测序反应外，核心服务还包括 准备各种DNA模板，并协助客户进行所有 DNA测序和分析的技术方面。核心也准备好 使用客户准备的库的高密度过滤器阵列。除了……之外 服务职能，核心人员报告基本DNA有所改善 测序化学，允许更长、更准确的读取长度和 改进了突变检测。促进中央大学的研究工作 遗传性癌症诊所，核心现在提供BRCA1/2筛查 185delAG、5382insC和6174delT突变。尤其是由于 基因组计划，对DNA测序的需求将继续扩大 意义重大。在下一个支助期内，核心计划打算继续 它为癌症中心成员提供最先进的服务。以研究为基础 突变/多态筛查也有可能扩大。在过去4年中 测序的年份样本从1501个增加到9410个(526%)，并收取 补缴费用由43,095元增至187,136元(334%)。因为 我们已经能够大幅提高收费，这导致了继续 核心使用率增加。核心已经被10个国家中的9个成员使用 癌症中心项目。核心将搬到菲茨西蒙斯园区， 癌症中心在下一个赠款周期内。