CORE--DNA SEQUENCING AND ANALYSIS

核心——DNA测序与分析

• 批准号: 6589975
• 负责人: HARRY A. DRABKIN
• 金额: $ 25.04万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6589975
• 关键词: DNA; biomedical facility; computer assisted sequence analysis; nucleic acid sequence

Description: (Applicant's Description) The DNA Sequencing and Analysis Core is now in its 4th year of highly successful operation. Initiated as a fledging operation after the inception of the CU Cancer Center, the Core has grown remarkably with now 112 user groups, of which most are Cancer Center members. In addition to performing DNA sequencing reactions, Core services include preparation of various DNA templates and assisting customers with all technical aspects of DNA sequencing and analysis. The Core also prepares high-density filter arrays using customer prepared libraries. In addition to service functions, Core personnel reported improvements in basic DNA sequencing chemistry that permit longer, more accurate read-lengths and improved mutation detection. To facilitate research efforts of the CU Hereditary Cancer Clinic, the Core now offers screening for the BRCA1/2 185delAG, 5382insC and 6174delT mutations. Especially as a consequence of the Genome Project, the demand for DNA sequencing will continue to expand significantly. During the next period of support, the Core intends to continue its state-of-the-art services to Cancer Center members. Research-based mutation/polymorphism screening is also likely to expand. Over the past 4 years samples sequenced have increased from 1501 to 9410 (526%) and charge back fees have increased from $43,095 to $187,136 (334%). Because of the large increase we have been able to reduce fees which has led to continued increases in core usage. The core has been used by members of 9 of the 10 Cancer Center programs. The core will move to the Fitzsimons campus with the Cancer Center during the next grant cycle.

描述：（申请人描述）DNA测序和分析核心 现已进入非常成功运营的第四个年头。作为一个羽翼未丰的 CU癌症中心成立后，Core不断发展壮大 值得注意的是，现在有112个用户组，其中大多数是癌症中心的成员。 除了进行DNA测序反应，核心服务包括 准备各种DNA模板，并协助客户进行所有 DNA测序和分析的技术方面。核心还准备 高密度过滤器阵列使用客户准备的库。除了 核心人员报告说，在基本的DNA方面， 测序化学允许更长、更准确的读取长度， 改进的突变检测。促进大学的研究工作 遗传性癌症诊所，核心现在提供BRCA 1/2筛查 185 delAG、5382 insC和6174 delT突变。特别是由于 基因组计划，对DNA测序的需求将继续扩大 显著在下一个支持期间，核心打算继续 为癌症中心成员提供最先进的服务。研究性 突变/多态性筛查也可能扩大。在过去4 年的样品测序从1501个增加到9410个（526%）， 补缴费用从43，095美元增加到187，136美元（334%）。因为 大幅增加，我们已经能够降低费用，这导致继续 核心使用量的增加。10个国家中有9个国家使用了核心 癌症中心计划。核心将搬到菲茨西蒙斯校园， 癌症中心在下一个赠款周期。