MOLECULAR AND STRUCTURAL BIOLOGY PROGRAM

描述：(申请者描述)分子与结构生物学 该计划汇集了23名杰出的调查人员，代表10名 3个UCHSC学院(医学、药学和研究生院)， 和2个姊妹机构(UCHSC和CU-Boulder)。科学的和 该计划的方案目标是阐明关键的基本过程 参与癌症生物学，并扩大分子结构成分 该计划分别为。我们在开发结构性产品方面的巨大成功 生物学倡议已将我们的计划扩展到基础知识的前沿领域 科学。该计划已重新组织，成员现在分为 三大看点：核酸代谢、基因转录 和分子结构。这组资金非常充足的调查人员(6.3美元 百万美元；直接成本)为分子机制提供了关键的见解 调控：DNA复制、修复和重排；RNA反式剪接 和信使核糖核酸的加工；阶段、细胞和信号特异的基因转录； 在阐明DNA、RNA、蛋白质和药物的化学结构方面 分子。在过去的五年里，我们的计划有了戏剧性的增长 在总支持(23%)、NCI支持(537%)和其他癌症支持(254%)中， 显示出显著的进步。此外，成员们还发布了超过 在备受尊敬的期刊上发表了200篇报道，突显了全国的显赫地位 项目教职员工。计划成员通过几种机制进行互动，包括 每月研讨会、年度静修、年度区域专题会议和年度 小型研讨会。这些正式的交互机制提供了多个 交流信息和合作努力的机会。这个 计划大量使用DNA测序、组织培养/克隆 以抗体、蛋白质化学和流式细胞术为核心。我们未来的计划是 进一步发展分子结构小组，特别是在 蛋白质组学，并通过以下方式提供对分子机制的关键见解 DNA复制机制、转录装置、 而RNA加工成分会导致癌症。

Description: (Applicant's Description) The Molecular and Structural Biology Program brings together 23 outstanding investigators representing 10 departments, 3 UCHSC Schools (Medicine, Pharmacy, and the Graduate school), and 2 sister institutions (UCHSC & CU-Boulder). The scientific and programmatic goals of the Program are to elucidate key fundamental processes involved in cancer biology and to expand the molecular structure component of the Program, respectively. Our tremendous success in developing a Structural Biology initiative has broadened our Program into cutting-edge areas of basic science. The Program has been re-organized and members are now divided into three focal points of strength: Nucleic Acid Metabolism, Gene Transcription and Molecular Structure. This very well funded group of investigators ($6.3 million; direct costs) has provided key insights into molecular mechanisms regulating: DNA replication, repair, and re-arrangements; RNA trans-splicing and mRNA processing; stage-, cell- and signal-specific gene transcription; and in elucidating the chemical structures of DNA, RNA, protein and drug molecules. During the past five years our Program has had dramatic increases in total support (23%), NCI support (537%), and other Cancer Support (254%), showing remarkable progress. Additionally, members have published more than 200 reports in highly respected journals, underscoring the national prominence of Program faculty. Program members interact via several mechanisms, including monthly seminars, annual retreat, annual regional topical meetings, and annual mini-symposium. These formal mechanisms of interaction provide multiple opportunities for exchange of information and collaborative efforts. The Program makes heavy use of the DNA Sequencing, Tissue Culture/Monoclonal Antibody, Protein Chemistry and Flow Cytometry Cores. Our future plans are to further develop the Molecular Structure group, particularly in the area of proteomics, and to provide critical insights into the molecular mechanisms by which aberrations of the DNA replication machinery, transcription apparatus, and RNA processing components lead to cancer.