CORE--VECTOR PRODUCTION

核心——矢量制作

• 批准号: 6652845
• 负责人: Arthur Dusty Miller
• 金额: $ 20.94万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652845
• 关键词: Retroviridae; biomedical facility; biotechnology; gene therapy; transfection /expression vector

The overall function of the Vector Core is to provide high quality retroviral vectors, including lentiviral vectors, to investigators of this SCOR grant. Many of the projects in this SCOR proposal utilize retroviral vectors as experimental tools to introduce specific genes into hematopoietic cells and other cultured cells. The Vector Core will provide different vector plasmids and packaging cell lines as well as inappropriate technical support relating to the best vectors to use for specific studies. The Vector Core will provide large quantities of vectors required for the baboon studies in Project 6, and will produce these vectors in a dedicated facility under conditions similar to those used for production of vectors for human use. The facilities of the Vector Core can be used by other investigators or Vector Core staff to generate vector-producing cells suitable for human use. Issues of the potential for production of replication-competent viruses will be addressed, and assays for such viruses will be performed for clinical trials and for vectors used in laboratory studies. Safety or toxicity assays that cannot be performed in the Core will be contracted to outside laboratories as needed.

载体核心的总体功能是向本次 SCOR 资助的研究人员提供高质量的逆转录病毒载体，包括慢病毒载体。该 SCOR 提案中的许多项目利用逆转录病毒载体作为实验工具，将特定基因引入造血细胞和其他培养细胞。载体核心将提供不同的载体质粒和包装细胞系，以及与用于特定研究的最佳载体相关的不适当的技术支持。 Vector Core将提供项目6中狒狒研究所需的大量载体，并将在专用设施中在类似于生产人类用载体的条件下生产这些载体。其他研究人员或 Vector Core 工作人员可以使用 Vector Core 的设施来生成适合人类使用的载体产生细胞。将解决生产具有复制能力的病毒的潜力问题，并将对此类病毒进行分析以用于临床试验和实验室研究中使用的载体。 无法在核心内进行的安全性或毒性测定将根据需要外包给外部实验室。