CORE--IMAGING

目得： 成像核心将具有2项服务功能。第一个功能是 为最先进的形态学方法提供技术支持。的 第二个功能是从尸体解剖中收集和评估人体组织， 手术切除和移植手术。 1.为最先进的形态学提供技术专长 方法.这些方法包括常规和专业定量 组织学和电子显微镜、免疫组织化学和原位 杂交方法我们在体内实验方面有相当的专业知识， 动物研究、体内实验和人体组织分析。 2.从尸体解剖、手术切除和 移植手术成像核心的第二个功能是 利用艾伯丁博士建立的人体组织库， 齐默尔曼医生的宝贵帮助迈克尔和麦金太尔等 迫切需要对ARDS患者的肺进行研究， 通过测量支气管肺泡灌洗和血浆样品中的因子 并作为免疫组织化学和其他研究的“金标准” 在急性肺损伤的动物模型中，如其他人所认识到的（Pittet et et 例如，1997年）。我们对未来的计划感到特别兴奋， 分析超出了具体项目的直接目标，一旦 已经定义了原位内皮细胞的炎性表型， 分析信号传导因子，表面粘附分子， 以及炎症环境中其他细胞类型中的其他关键变量 肺在ARDS。令人兴奋的未来计划之一是分享我们的人类 组织库与其他SCOR计划，如我们正在计划与博士。 哈德逊在西雅图的SCOR小组。 重要性： 成像核心的贡献将大大提高我们的 了解失调的细胞间相互作用和细胞间 在ARDS的不同阶段的信令（例如，早期与晚期损伤， 损伤与修复）。

PURPOSE: The Imaging Core will have 2 service functions. The first function is to provide technical support for state-of-the-art morphological methods. The second function is to collect and evaluate human tissue from autopsies, surgical resections and transplant procedures. 1. To provide technical expertise for state-of-the-art morphological methods. These methods include routine and specialized quantitative histology and electron microscopy, immunohistochemistry, and in situ hybridization. We have considerable expertise with in vivo experimental animal studies, in vivo experiments, and human tissue analyses. 2. To collect human tissues from autopsies, surgical resections and transplant procedures. The second function of the Imaging Core is to exploit the human tissue bank that Dr. Albertine established, with the invaluable assistance of Drs. Zimmerman. Michael and McIntyre. Such studies of the human lung in the ARDS are desperately needed to correlate with measurements of factors in bronchoalveolar lavage and plasma samples and to use as a "gold standard" for immunohistochemical and other studies in animal models of acute lung injury, as recognized by others (Pittet et al., 1997). We are particularly excited by the future plan of extending the analysis beyond the immediate goals of the specific projects, once the inflammatory phenotype of in situ endothelial cells has been defined, to analysis of the display of signaling factors, surface adhesion molecules, and other key variable sin other cell types in the milieu of the inflamed lung in ARDS. One of the exciting future plans is to share our human tissue bank with other SCOR programs, such as we are planning with Dr. Hudson's SCOR group in Seattle. SIGNIFICANCE: The contributions of the Imaging Core will substantially improve our understanding of dysregulated cell-cell interactions and intercellular signaling at different stages of the ARDS (e.g., early versus late injury, and injury versus repair).