DRUG ABUSE RESEARCH CENTER IN MOLECULAR AND CELL BIOLOGY

为了同步两个现有的计划项目赠款，e建议 建立药物滥用基础分子和细胞研究中心 明尼苏达大学的生物学，利用 药理学系的核心教师，更有效地促进 服务和互动与其他药物滥用研究人员在这方面 社区在过去的8年里，我们已经证明，两个P01赠款 作为一个单一的项目，与贺拉斯一起成功地、连贯地工作， H.洛饰演私家侦探因此，在目前的提案中，我们结合了各种 这两个项目的组成部分，以建立拟议的药物 虐待研究中心。拟议中心的主要目标是：（a） * 在药物滥用研究中促进跨学科方法， （B）作为一个“活动”中心，协调和 促进药物滥用研究方面的所有学术活动， 明尼阿波利斯大都市区;（c）作为国家资源， 药物滥用机制的分子和细胞研究， 提供专业知识、试剂、克隆细胞系或转基因/敲除 （d）向本地及全国的滥用药物人士提供动物;及 作为明尼苏达州年轻科学家的培训中心内 在拟议的中心，将有一个行政核心， 分子/细胞和遗传核心，抗体生产核心和 生物成像核心，将支持科学活动， 件.共有8个科学组成部分，5个组成部分 直接处理毒品鸦片制剂的不同方面， 其他三个涉及其他滥用药物，大麻，尼古丁和 咖啡因所有这8个组成部分处理的调查的基本 这些药物滥用的分子和细胞生物学机制。的 使用的不同方法：转基因动物、分子生物学 蛋白质和基因的突变分析，第二信使调节， 电生理测量和转录控制研究将 为培养青年科学家提供了肥沃的土壤， 在调查人员和开发资源的药物滥用研究 社区在当地和全国。

In order to synchronize the two exiting program project grants, e propose to establish a Drug Abuse Research Center in Basic Molecular and Cell Biology at University of Minnesota which utilizes the expertise of the core faculty in the Department of Pharmacology and more effective promotes service and interaction with other drug abuse researchers in this community. During the past 8 years, we have proven that two P01 grants have worked successfully and coherently as a single program, with Horace H. Loh as the P.I. Thus, in the current proposal, we have combined various components from these two program projects to establish the proposed Drug Abuse Research Center. The main objectives of the proposed center are: (a) foster interdisciplinary approaches in drug abuse research among the investigators; (b) to serve as an "activity" center to coordinate and to promote all academic and scholarly activities in drug abuse research in the Minneapolis metropolitan area; (c) to serve as a national resource for molecular and cellular studies in the mechanisms of drug abuse either by providing expertise, reagents, clonal cell lines or transgenic/knockout animals to the drug abuse community locally and nationally; and (d) to serve as training center for young scientists here in Minnesota. Within the proposed center, there will be an Administration Core, a Molecular/Cellular and Genetic Core, an Antibodies Production Core and a Bioimaging Core which will support the activities of the scientific components. There are total 8 scientific components with 5 components which deal directly with the different aspects of the drug opiate, while the other three deals with other drugs of abuse, marijuana, nicotine and caffeine. All these 8 components deal with the investigation of the basic molecular and cell biological mechanisms of these drugs of abuse. The different approaches used: transgenic animals, molecular biological mutational analysis of proteins and genes, second messengers regulations, electrophysiological measurements and transcription control studies will provide a fertile ground for training of young scientists, interaction among investigators nd developing resource for the drug abuse research community locally and nationally.