Core--Genetic analysis

描述(取自应用程序) 西奈山超级基金基础研究计划基因分析核心 E，将进行生物医学所需的所有基于核酸的分析 研究项目10和3。核心将提取和存储编码的人类DNA 用于基因分析的样本。双等位基因将通过聚合酶链式反应-RFLP确定 (聚合酶链式反应-限制性片段长度多态性)或 等位基因特异性聚合酶链式反应。相互竞争的技术可能会取代成本 还原。这个核心的工作将决定和描述人类 已知的影响铅代谢的基因多态[ALAD、VDR和HFE]。另外， CORE将研究影响有机氯代谢的多态 可能有助于基因-环境相互作用，潜在地与 帕金森氏病的起源[CYP2D6，CYP2E1，PON1和GSTP1，以及 PON2和PON3已知的和新衍生的多态]。其他内容 多态将根据研究项目的要求进行确定 董事们。核心将确定小鼠Wnt4，Wnt5a和WNT7a的基因类型 基因敲除小鼠杂交后代支持研究项目9。 CORE将为三个小鼠WNT基因寻找人类WNT多态 在项目9中学习。也将调查人类PON_2和PON_3。《核心》 将根据董事的要求搜索其他人类多态 研究项目的一部分。

DESCRIPTION (Taken from application) The Mount Sinai Superfund Basic Research Program Genetic Analysis Core, Core E, will carry out all nucleic acid based assays required for the biomedical Research Projects 10 and 3. The Core will extract and store coded human DNA samples for genotype analyses. Biallelic genotypes will be determined by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) or allele-specific PCR. Competing technologies may be substituted for cost reduction. The work of this Core will determine and characterize human polymorphisms known to affect lead metabolism [ALAD, VDR and HFE]. Also, the Core will examine polymorphisms affecting metabolism of organochlorines that may contribute gene-environment interactions potentially relevant to the genesis of Parkinson's Disease [CYP2D6, CYP2E1, PON1 and GSTP1, as well as known and newly-derived polymorphisms in PON2 and PON3]. Additional polymorphisms will be determined as requested by the Research Project Directors. The Core will determine mouse WNT4, WNT5a and WNT7a genotypes of progeny in crosses of knock-out mice in support of Research Project 9. The Core will search for human WNT polymorphisms for the three mouse WNT genes studied in Project 9. Human PON2 and PON3 will also be investigated. The Core will search for additional human polymorphisms as requested by the Directors of the Research Projects.