High Density Printing for Mt Sinai Microarray Facility

这项提案的总体目标是获得一个高容量的阵列，BioRobotics MicroGrid II TAS系统，使我们新开发的机构微阵列共享研究设施，以生产最先进的cDNA微阵列与20，000甚至高达40，000的功能。该微阵列设施于2000年8月获得批准。微阵列设备经理于2001年1月招聘并开始工作。600平方米的翻新英尺分配给微阵列设施的克隆库已接近完成，另外的克隆库以及用于储存和加工成可打印的PCR产品的主要设备已订购并已开始运抵。通过西奈山的教职员工早期发起的努力，我们已经证明了生产具有多达3000个特征的可重复的高质量cDNA阵列的能力。由于我们现有的GMS 417点样器的打印能力有限，打印具有3000个特征的载玻片需要巨大的努力。除了速度慢之外，由于其死体积，该阵列器需要过量的纯化PCR产物。随着所要求的仪器和西奈山的机构承诺或空间，技术援助和服务合同的支持，吞吐量将大大增加，导致成本效益的微阵列设施。

The overall objective of this proposal is to obtain a high-capacity arrayer, the BioRobotics MicroGrid II TAS System, to enable our newly-developed Institutional Microarray Shared Research Facility to produce state-of-the-art cDNA microarrays with 20,000 or even up to 40,000 features. This Microarray Facility was approved in August 2000. The Microarray Facility Manager was recruited and began work in January, 2001. Renovation of the 600 sq. ft. assigned to the Microarray Facility is nearly complete, and additional clone banks and major items of equipment for their storage and processing into printable PCR products have been ordered and have begun to arrive. Through efforts initiated earlier by Mount Sinai faculty and staff, we have demonstrated the capacity to produce reproducible, high-quality cDNA arrays with up to 3000 features. Printing slides with 3000 features requires a Herculean effort due to the limited printing capacity of our existing GMS 417 arrayer. In addition to slow speed, this arrayer requires excessive quantities of purified PCR products because of its dead volume. With the requested instrumentation and Mount Sinai's institutional commitment or space, technical assistance, and service contract support, throughput will be greatly increased leading to a cost- effective Microarray Facility.