High Density Printing for Mt Sinai Microarray Facility

本提案的总体目标是获得一个高容量阵列，即BioRobotics MicroGrid II TAS系统，以使我们新开发的机构微阵列共享研究设施能够生产具有20,000甚至高达40,000个特征的最先进的cDNA微阵列。该微阵列设备于2000年8月获得批准。Microarray设备经理于2001年1月被招聘并开始工作。600平方米的翻新。分配给微阵列设施的第1部分已接近完成，用于储存和加工成可打印PCR产品的额外克隆库和主要设备项目已订购并开始到达。通过西奈山教职员工早些时候发起的努力，我们已经展示了生产可重复的高质量cDNA阵列的能力，其中包含多达3000个特征。由于我们现有的GMS 417阵列的打印能力有限，打印具有3000个特征的幻灯片需要付出巨大的努力。除了速度慢外，由于其死体积，该阵列需要大量纯化的PCR产物。有了所要求的仪器和Mount Sinai的机构承诺或空间、技术援助和服务合同支持，吞吐量将大大增加，从而实现成本效益高的微阵列设备。

The overall objective of this proposal is to obtain a high-capacity arrayer, the BioRobotics MicroGrid II TAS System, to enable our newly-developed Institutional Microarray Shared Research Facility to produce state-of-the-art cDNA microarrays with 20,000 or even up to 40,000 features. This Microarray Facility was approved in August 2000. The Microarray Facility Manager was recruited and began work in January, 2001. Renovation of the 600 sq. ft. assigned to the Microarray Facility is nearly complete, and additional clone banks and major items of equipment for their storage and processing into printable PCR products have been ordered and have begun to arrive. Through efforts initiated earlier by Mount Sinai faculty and staff, we have demonstrated the capacity to produce reproducible, high-quality cDNA arrays with up to 3000 features. Printing slides with 3000 features requires a Herculean effort due to the limited printing capacity of our existing GMS 417 arrayer. In addition to slow speed, this arrayer requires excessive quantities of purified PCR products because of its dead volume. With the requested instrumentation and Mount Sinai's institutional commitment or space, technical assistance, and service contract support, throughput will be greatly increased leading to a cost- effective Microarray Facility.