Environmental stress indicators for fish at Superfund sites

超级基金地点鱼类的环境压力指标

• 批准号: 6613356
• 负责人: DONALD C MALINS
• 金额: $ 22.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6613356
• 关键词: DNA damage; aquatic organism; aromatic hydrocarbon receptor; biomarker; biopsy; cytochrome P450; ecosystem; environmental contamination; environmental toxicology; field study; fish; gas chromatography mass spectrometry; gills; hazardous substances; histopathology; infrared spectrometry; marine biology; method development; model design /development; soil sampling; water environment; water pollution

The goal of the proposed work is to employ a sequence of research steps leading to a model for the use of non-destructive gill biopsies of fish to monitor marine contamination at Superfund sites and identify changes in the health status of this resource. The results would provide a significant understanding of relationships between environmental contamination histology and a group of discerning biomarkers relating to fish health. The specific aims are: 1) collect fish and marine sediments from contaminated locations and uncontaminated reference areas in Puget Sound, WA (English sole) and the San Francisco Bay Area (surf perch), areas that have a wide spread of contaminant burdens and biological impacts; 2) measure the aryl hydrogen (Ah)-inducing contamination in marine sediments using the P450 Reporter Gene System (RGS) assay, perform histological examination of liver, kidney and gill tissues and analyze DNA from each tissue using gas chromatography-mass spectrometry (GC0MS) and Fourier transform-infrared (FT-IR) spectroscopy. Complementary cytochrome P450 1A (CYP1A) and nuclear factor kappa B (NfkappaB) models would be employed in conjunction with the DNA models; 3) determine the association of the histology of biomarker data with the level of sediment contamination and P4501A induction. Then determine whether the biological indicators applied to ecologically non-destructive gill tissues and P4501A induction. Then determine whether the biological indicators applied to ecologically non-destructive gill tissues could be used alone for the assessment of fish health at Superfund sites and 4) determine if the association between sediment contamination, histology and the biomarkers are similar between English sole and serf perch to see if the use of the biological indicators is independent of fish species. Alternative, if species differences are a factor, establish a health-related model that takes this into account.

拟议工作的目标是采用一系列研究步骤，形成一个模型，利用非破坏性的鱼类鳃活检来监测超级基金地点的海洋污染，并确定这种资源健康状况的变化。该结果将为环境污染组织学和一组与鱼类健康相关的生物标志物之间的关系提供重要的理解。具体目标是：1)收集受污染地点和未受污染参考区域的鱼类和海洋沉积物，西澳普吉特海湾（英底）和旧金山湾区（冲浪鲈），这些地区有广泛的污染物负担和生物影响；2)使用P450报告基因系统（RGS）测定海洋沉积物中芳香氢（Ah）诱导污染，对肝脏、肾脏和鳃组织进行组织学检查，并使用气相色谱-质谱（GC0MS）和傅里叶变换-红外（FT-IR）光谱分析每个组织的DNA。互补细胞色素P450 1A （CYP1A）和核因子κ B （NfkappaB）模型将与DNA模型结合使用；3)确定生物标志物组织学数据与沉积物污染水平和P4501A诱导的关系。然后确定生物指标是否适用于生态无损的鳃组织和P4501A诱导。然后确定应用于生态非破坏性鳃组织的生物指标是否可以单独用于超级基金站点的鱼类健康评估；4)确定沉积物污染、组织学和生物标志物之间的关联是否相似，以确定生物指标的使用是否独立于鱼类。另一种选择是，如果物种差异是一个因素，则建立一个考虑到这一点的健康相关模型。