Environmental stress indicators for fish at Superfund sites

拟议工作的目标是采用一系列研究步骤，建立一个使用非破坏性鱼鳃活组织检查的模型，以监测超级基金地点的海洋污染，并确定这一资源的健康状况的变化。这些结果将为环境污染组织学和一组与鱼类健康相关的识别生物标志物之间的关系提供重要的理解。具体目标是：1）从华盛顿州普吉特湾的污染地点和未污染参考区收集鱼类和海洋沉积物（英国唯一）和旧金山弗朗西斯科湾区（冲浪鲈鱼），具有广泛的污染物负担和生物影响的地区; 2）用P450报告基因系统（RGS）检测海洋沉积物中芳基氢（Ah）诱导的污染，进行肝脏组织学检查，肾脏和鳃组织，并使用气相色谱-质谱（GCMS）和傅立叶变换-红外（FT-IR）光谱分析来自每个组织的DNA。补充细胞色素P450 1A（CYP 1A）和核因子κ B（NfkappaB）模型将与DNA模型结合使用; 3）确定生物标志物数据的组织学与沉积物污染水平和P4501 A诱导的相关性。然后确定该生物指示剂是否适用于对生态无破坏的鳃组织和P4501 A的诱导。然后确定是否可以单独使用的生物指示剂应用于生态非破坏性鳃组织的鱼类健康的评估在超级基金网站和4）确定沉积物污染，组织学和生物标志物之间的关联是否是类似的英国唯一和农奴鲈鱼，看看生物指示剂的使用是独立的鱼类物种。另一种方法是，如果物种差异是一个因素，建立一个考虑到这一点的健康相关模型。

The goal of the proposed work is to employ a sequence of research steps leading to a model for the use of non-destructive gill biopsies of fish to monitor marine contamination at Superfund sites and identify changes in the health status of this resource. The results would provide a significant understanding of relationships between environmental contamination histology and a group of discerning biomarkers relating to fish health. The specific aims are: 1) collect fish and marine sediments from contaminated locations and uncontaminated reference areas in Puget Sound, WA (English sole) and the San Francisco Bay Area (surf perch), areas that have a wide spread of contaminant burdens and biological impacts; 2) measure the aryl hydrogen (Ah)-inducing contamination in marine sediments using the P450 Reporter Gene System (RGS) assay, perform histological examination of liver, kidney and gill tissues and analyze DNA from each tissue using gas chromatography-mass spectrometry (GC0MS) and Fourier transform-infrared (FT-IR) spectroscopy. Complementary cytochrome P450 1A (CYP1A) and nuclear factor kappa B (NfkappaB) models would be employed in conjunction with the DNA models; 3) determine the association of the histology of biomarker data with the level of sediment contamination and P4501A induction. Then determine whether the biological indicators applied to ecologically non-destructive gill tissues and P4501A induction. Then determine whether the biological indicators applied to ecologically non-destructive gill tissues could be used alone for the assessment of fish health at Superfund sites and 4) determine if the association between sediment contamination, histology and the biomarkers are similar between English sole and serf perch to see if the use of the biological indicators is independent of fish species. Alternative, if species differences are a factor, establish a health-related model that takes this into account.