Using genomic and postgenomic tools to identify the geomicrobiological basis of arsenic mobilisation in aquifers

使用基因组和后基因组工具确定含水层中砷迁移的地球微生物学基础

• 批准号: 2108298
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2108298
• 关键词: Using; genomic; postgenomic; tools; identify

As part of a major collaborative international programme with other leading arsenic groups, sediment cores and groundwaters will be obtained from aquifers with high arsenic concentrations, using state of the art sampling procedures and preserved for detailed analyses in Manchester. Molecular ecology studies will be conducted, including phylogenetic and functional profiling of microbial communities using illumine sequencing of 16S rRNA or functional gene amplicons. The student will also develop and apply metagenomic, transcriptomic and proteomic approaches for analyses of these samples, to help elucidate the in situ mechanism of arsenic mobilization. Parallel mineralogical and geochemical analyses will be conducted on these samples, and in carefully-designed microcosm studies to support this work, and help build a comprehensive model for the mobilisation of arsenic in aquifer sediments. The student will work under the supervision of a team of geomicrobiologists, geochemists and mineralogists and will be part of a group of academic and technical staff, postdocs and PhD students working on subsurface microbiology and arsenic biogeochemistry at Manchester. They will be based in state of the art laboratories in the Williamson Research Centre for Molecular Environmental Science, and will also benefit from the excellent infrastructure for post-genomic science in the Faculty of Life Sciences. The student will generate a series of high impact publications and will be well prepared for a career in molecular environmental science.References (1) Islam, F.S., Gault, A.G., Boothman, C., Polya, D.A., Charnock, J.M., Chatterjee, D. and Lloyd, J.R. (2004) Role of metal-reducing bacteria in arsenic release from Bengal Delta sediments. Nature 430 68-71. (2) Lear, G., Song, B., Gault, A.G., Polya, D.A. and J.R. Lloyd (2007) Molecular Analysis of Arsenate-Reducing Bacteria within Cambodian Sediments Following Amendment with Acetate. Applied and Environmental Microbiology 731041-104. (3) Handley, K.M., McBeth, J.M., Charnock, J.M., Vaughan, D.J., Wincott, P.L., Polya, D.A. and Lloyd, J.R. (2013) Effect of iron redox transformations on arsenic solid-phase associations in an asenic-rich, ferruginous hydrothermal sediment. Geochimica et Cosmochimica Acta 102 124-142

作为与其他主要砷集团合作的重大国际方案的一部分，将使用最先进的采样程序从高砷含量的含水层获取沉积物岩芯和地下水，并保存在曼彻斯特进行详细分析。将进行分子生态学研究，包括利用16S rRNA或功能基因扩增的光照测序对微生物群落进行系统发育和功能分析。学生还将开发和应用后基因组学、转录组学和蛋白质组学方法来分析这些样本，以帮助阐明砷动员的原位机制。将对这些样品进行平行的矿物学和地球化学分析，并在精心设计的微观研究中支持这项工作，并帮助建立一个全面的含水层沉积物中砷的动员模型。这名学生将在一个由地质微生物学家、地球化学家和矿物学家组成的团队的指导下工作，并将成为曼彻斯特地下微生物学和砷生物地球化学研究小组中学术和技术人员、博士后和博士后的一员。他们的基地将设在威廉姆森分子环境科学研究中心的最先进的实验室，还将受益于生命科学学院出色的后基因组科学基础设施。参考文献(1)伊斯拉姆，F.S.，Gault，A.G.，Boothman，C.，Polya，D.A.，Charnock，J.M.，Chatterjee，D.和Lloyd，J.R.(2004)金属还原细菌在孟加拉三角洲沉积物砷释放中的作用。大自然430 68-71。(2)Lear，G.，Song，B.，Gault，A.G.，Polya，D.A.和J.R.Lloyd(2007)经乙酸盐修正后柬埔寨沉积物中的砷还原细菌的分子分析。应用与环境微生物学731041-104年。(3)Handley，K.M.，McBeth，J.M.，Charnock，J.M.，Vaughan，D.J.，Wincott，P.L.，Polya，D.A.和Lloyd，J.R.(2013)铁的氧化还原转化对富含铁的热液沉积物中砷的固相组合的影响。地学与宇宙学报102 124-142