Core--Imaging

成像核心设施将在光学显微镜成像和分析领域为该计划提供服务。核心是建立在国家的最先进的设施，在医学部肾脏科，并受益于关键核心人员的经验，在应用成像方法分析造血细胞功能。该设施目前配备了两个共焦激光扫描显微镜：蔡司LSM 510能够紫外激发荧光;和BioRad MRC-1024与钛：蓝宝石激光器能够2光子激发以及传统的共焦成像的Kr/Ar激光。两个配备冷却CCD相机的宽视场显微镜支架可供选择：一个应用精密Deltavision系统优化图像去卷积;第二个尼康支架用于运动分析。带CO2灌注的载物台培养箱可用于在显微镜载物台上长时间保持活细胞并对其成像。 现有的计算资源包括装有图像处理、图像反卷积、三维图像绘制和图像数据定量分析软件的工作站。成像核心的目的是提供1）通过透射光显微镜和荧光显微镜成像活造血细胞的技术和专业知识，以促进信号事件和Rac 2依赖过程的实时分析;和2）荧光显微镜生成的图像数据的定量分析的专业知识和支持，以补充造血细胞功能的生化和其他分析。

The Imaging Core facility will service the program in the area of light microscope imaging and analysis. The core is built around the state-of- the-art facility available in the Nephrology Division of the Department of Medicine, and benefits from the experience of key core personnel in the application of imaging methodologies to the analysis of hematopoietic cell function. The facility is currently equipped with two confocal laser-scanning microscopes: a Zeiss LSM 510 capable of UV excitation of fluorescence; and a BioRad MRC-1024 with Ti: sapphire laser capable of 2-photon excitation as well as Kr/Ar laser for conventional confocal imaging. Two wide-field microscope stands equipped with cooled CCD cameras are available: an Applied Precision Deltavision system optimized for image deconvolution; and a second Nikon stand used for motility assays. Stage incubators with CO2 perfusion are available for maintaining and imaging live cells on the stage of the microscope for long periods. Computing resources available include workstations with software for image processing, image deconvolution, rendering of 3-D image volumes and quantitative analysis of image data. The aim of the imaging core is to provide 1) technology and expertise in imaging live hematopoietic cells by transmission light microscopy and fluorescence microscopy to facilitate real-time analysis of signaling events and Rac2 dependent processes; and 2) expertise and support for quantitative analysis of image data generated by fluorescence microscopy to complement biochemical and other analysis of hematopoietic cell function.