CORE--TRANSGENIC MOUSE

转基因小鼠核心的目的是提供两个关键 为项目成员提供科学支持服务。 核心 将与首席研究员密切合作， 具体的PO1项目，提供专业知识和设备，以生产 转基因小鼠 在某些项目中，内核会使用一种特殊的基因 做转基因的背景。 其次，核心将提供 服务，以产生空突变小鼠，再次密切合作， 项目调查员。 与其他常用的核心支持不同， 服务，我们已决定组织这项服务的“即付即用” 基础，即仅要求资金用于维护核心模型 每个项目的项目建议将是 大部分是由工作人员产生的，并将由具体的 项目 在一些不同的指导和建议， 将提供产生小鼠模型所需的技术， 核心人员。 此外，核心小组支付的Res.Spec.II 将执行这些过程中的关键步骤（例如，生长ES 细胞，显微注射）。 本组织的主要考虑因素 结构是一方面，大量的“动手”时间 个别项目所需的参与，例如， 一只空突变小鼠，通常需要比平常更多的时间和精力， 正常的工作日。 另一方面， 关键的步骤只有由一个完全 致力于这种技术并在这方面经验丰富。 小鼠模型将具有 将在项目1（Minoo博士）、项目2（Warburton博士）和 项目4（Groffen博士）。 基于我们最近的几个项目 澄清会议，很可能项目3（DeRynch博士）将 需要开发小鼠模型（空突变和转基因）。 的 核心领导人（Heisterkamp博士），他在 这两种类型的小鼠模型的产生，将严格评估 设计每一个结构，并将参与教学 人员的

The purpose of the Transgenic Mouse Core is to provide two key scientific support services to members of the Program Project. The core will, in close collaboration with the principal investigator of a specific PO1 project, provide the expertise and equipment to produce transgenic mice. For some projects, the Core will use a special genetic background to make transgenics. Secondly, the core will provide services to generate null-mutant mice, again in close collaboration with the project investigator. Unlike other commonly available core support services, we have decided to organize this service on a "pay-as-you-go" basis i.e. funds are requested only to maintain the coreouse models which the individual Project Project projects propose to make will be largely generated by personnel of and will be paid by that specific project. Instruction in and advice on some of the different technologies necessary to generate mouse models will be provided for by core personnel. In addition, the Res. Spec. II paid for by the Core will perform the critical steps in these procedures (e.g., growing ES cells, microinjection). A prime consideration for this organizational structure is on the one hand, the substantial "hands-on" time involvement required for an individual project, e.g. up to 1.5 years for a null-mutant mouse, often reuiring more time and effort than usual for a normal working day. On the other hand, it is very likely that critical steps will only be performed efficiently by someone fully dedicated to and experienced in such techniques. Mouse models will have to be generated inproject 1 (Dr. Minoo), project 2 (Dr. Warburton) and project 4 (Dr. Groffen). Based onseveral of our more recent project investigatory meetings, it is likely that project 3 (Dr. DeRynch) will have a need to develop mouse model(s) (null-mutant and transgenic). The Core leader (Dr. Heisterkamp) who has many years of experience in the generation of both types of mouse models, will critically evaluate the design of each construct and will be involved in the teaching of personnel.