Structure and function of the Clostridium botulinum spore

肉毒杆菌孢子的结构和功能

• 批准号: 2109109
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2109109
• 关键词: Structure; function; Clostridium; botulinum; spore

This is a cross-disciplinary collaboration building on recent work (Terry et al., 2017; Jiang et al., 2015; Janganan et al. 2016 - joint publication) and the award of a joint BBSRC grant to PAB and RF (2017). Both applicants have a strong track record in applying complementary approaches of imaging and molecular biology to bacterial cell surface structures. Our aim is to understand the mechanics of Clostridium botulinum spore assembly and differentiation into the vegetative cell. This is the form that produces the potentially lethal botulinum toxins. Spores are agents of food contamination and potential vehicles for bioterrorism. To achieve our aim, it is vital that we understand the 3D molecular architecture of the spore, and how it changes.

这是一项跨学科合作，建立在最近的工作（Terry 等人，2017 年；Jiang 等人，2015 年；Janganan 等人，2016 年 - 联合出版）以及向 PAB 和 RF 授予 BBSRC 联合资助（2017 年）的基础上。两位申请人在将成像和分子生物学互补方法应用于细菌细胞表面结构方面都有着良好的记录。我们的目的是了解肉毒杆菌孢子组装和分化为营养细胞的机制。这种形式会产生潜在致命的肉毒杆菌毒素。孢子是食品污染的媒介，也是生物恐怖主义的潜在载体。为了实现我们的目标，了解孢子的 3D 分子结构及其变化至关重要。