Instrumentation For High-Resolution Analysis of Cancer

癌症高分辨率分析仪器

• 批准号: 6584885
• 负责人: JOHN A ZEBALA
• 金额: $ 14.98万
• 依托单位: SYNTRIX BIOSYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-13 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6584885
• 关键词: biomedical automation; biomedical equipment development; biotechnology; laser capture microdissection; light microscopy; method development; molecular film; optics; photochemistry; sectioning; tissue /cell preparation

DESCRIPTION (provided by applicant): The analysis of cancer within complex heterogeneous tissues represents special challenges posed by the presence of infiltrating non-neoplastic cells. We have developed biolithography, a novel and proprietary method (U.S. Pat. No. 6,159,681 to Syntrix, Inc.) for high-resolution tissue microdissection that employs lithography directly on biologic samples. Biolithography offers several advantages over laser-based methods that include improvements in spatial resolution, reliability, and contamination risk. Importantly, there is the potential for simplified and less costly instrumentation because the method requires only low-energy incoherent light. In this Phase I/II FAST-TRACK proposal, we propose to develop an automated biolithography system that will integrate the advantages of biolithography within an automated system that offers both attractive performance and cost characteristics. The automated biolithography system will consist of a novel PhotoBlaster instrument that projects user-defined microscopic light patterns during biolithography using low-energy incoherent light and a silicon chip-based microdisplay device. Consumables will consist of adhesive polypropylene reaction wells and validated reagents and photofilms suitable for isolating DNA, RNA and protein from microscopic regions of interest. Phase I is designed to prove the feasibility of the proposed PhotoBlaster design to generate photopatterns of sufficient intensity and minimum feature size. The Phase II program will yield a final system prototype. The proposed automated system of biolithography offers the potential to provide a facile and cost-effective method to address whole organism analysis for a wide audience of researchers and clinicians engaged in cancer analysis. Syntrix will commercialize the PhotoBlaster system that results from this research either through direct sales or through third-parties.

描述（由申请人提供）： 复杂异质组织中癌症的分析代表了浸润性非肿瘤细胞的存在所带来的特殊挑战。我们已经开发了生物光刻，一种新颖的专有方法（美国专利号6，500，000）。美国专利号6，159，681）用于直接在生物样品上采用光刻的高分辨率组织显微切割。与基于激光的方法相比，生物光刻提供了几个优势，包括空间分辨率，可靠性和污染风险的改进。重要的是，有可能简化和成本较低的仪器，因为该方法只需要低能量的非相干光。在这个I/II期快速通道提案中，我们建议开发一种自动化生物光刻系统，该系统将生物光刻的优点集成在一个自动化系统中，该系统提供了具有吸引力的性能和成本特性。自动生物光刻系统将包括一种新型的PhotoBlaster仪器，该仪器在生物光刻过程中使用低能量非相干光和基于硅芯片的微显示设备投射用户定义的微观光图案。耗材将包括粘性聚丙烯反应威尔斯孔和经验证的试剂以及适用于从感兴趣的微观区域分离DNA、RNA和蛋白质的胶片。第一阶段旨在证明拟议的PhotoBlaster设计的可行性，以生成足够强度和最小特征尺寸的光图案。第二阶段计划将产生最终的系统原型。所提出的生物光刻自动化系统提供了一种简便和具有成本效益的方法，以解决整个生物体分析的广大受众的研究人员和临床医生从事癌症分析的潜力。Syntax将通过直接销售或通过第三方将这项研究产生的PhotoBlaster系统商业化。