O2 ELECTRODE PRODUCTION & DESIGN

氧气电极生产

• 批准号: 6568419
• 负责人: R P MALCHOW
• 金额: $ 21.13万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6568419
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical equipment development; biomedical resource; respiratory system; technology /technique

As part of the Center effort to develop and broaden the application of polarographic electrodes to the self-referencing modality we are beginning the production of our own electrodes. Although theoretically straight forward the mechanics of production are difficult. In brief a glass micro-electrode is filled with Woods Metal and etched to form a recess. The surface of the metal is coated with gold by electroplating and the remaining space filled or not with an 'oxygen-selective' membrane. Whether the membrane is used remains a matter of debate for our applications. In both the case of a large recess and a membrane, a main function is to reduce the oxygen depletion gradient at the tip of the electrode. This summer saw the first attempts at building our own electrodes. This work was done in collaboration with Paul Malchow with technical advice from Kim Mitchell (Center for Neurobiology and Immunology Research, University of Kansas), and Robert Linsenmeier and Lissa Padnick (both of Northwestern University, Chicago). In the latter case these investigators gifted the gold-plating solution. Electrodes have been successfully manufactured and applied to the study of oxygen consumption by Skate rod cells (see sub-project 21). Previous studies with commercially manufactured electrodes (Diamond General) produced similar results giving us a degree of confidence that our procedures are correct. The need to manufacture our own polarographic electrodes is driven largely by cost. Each electrode is priced at approximately $150 and our experience has demonstrated that only about one in five are quiet enough for our application. Presently, Diamond General are replacing all electrodes found to be below our needed standard. The additional advantage in producing our own electrodes is the ability to diversify the design as needed for the manufacture of nitric oxide detectors. This will be our next R&D step in this area where we hope to gain immensely from our consultative arrangement with Kim Mitchell and the recruitment to our advisory group of Thomas Goldstick.

作为中心努力发展和扩大 极谱电极在自参比中的应用 我们开始生产我们自己的电极。 虽然从理论上讲， 很难 简而言之，玻璃微电极填充有Woods 金属并蚀刻以形成凹部。 金属的表面涂有 通过电镀用金，剩余的空间填充或不填充 一种“氧选择性”膜。 膜是否被使用仍然是 这是我们申请的一个争议问题。 在这两种情况下， 凹部和膜，一个主要功能是减少氧气 电极尖端的耗尽梯度。 今年夏天， 第一次尝试制造我们自己的电极。 这项工作是在 与Paul Malchow合作，并获得Kim的技术建议 米切尔（神经生物学和免疫学研究中心，大学 堪萨斯）和罗伯特·林森迈尔（Robert Linsenmeier）和莉萨·帕德尼克（Lissa Padnick）（两人都是 西北大学，芝加哥）。 在后一种情况下，这些 调查人员赠送了镀金溶液。 电极已经 成功制造并应用于氧气的研究 Skate rod细胞的消耗（见分项目21）。 以前的研究 用商业制造的电极（Diamond General）生产 类似的结果给了我们一定程度的信心， 是正确的。 需要制造我们自己的极谱电极 很大程度上是由成本驱动的。 每个电极的价格约为 我们的经验表明，只有五分之一的人 对我们的申请来说足够安静了 目前，钻石将军 更换所有发现低于我们所需标准的电极。 的 生产我们自己的电极的另一个优点是能够 根据制造一氧化氮的需要使设计多样化 探测器 这将是我们在这一领域的下一个研发步骤，我们希望 从我们与金·米切尔的协商安排中获益匪浅 以及招募托马斯·戈德斯蒂克加入我们的顾问团