ANTIGEN RECOGNITION BY LYMPHOCYTES

淋巴细胞对抗原的识别

• 批准号: 6497236
• 负责人: Philippa C. Marrack
• 金额: $ 82.46万
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6497236
• 关键词: biological signal transduction; leukocyte activation /transformation; lymphocyte

Lymphocyte activation and tolerance continues to be an important topic of much theoretical and practical significance. The projects in this program are linked by a common interest in this subject. Overall, their goal is to find out how circumstances control the response, or lack of response, or a lymphocyte to antigen. This control is important both to our ability to generate efficient vaccines and to understand autoimmune disease. Experiments to be done in project 1 will deal with a new model of B cell tolerance in which self antigens make B cells non-responsive in such a way that their responsiveness can easily be regenerated. In project 2, the investigators will study the geography of the interface between thymocytes and tolerising or selecting ligand. The investigators will also study the interface between CD8+ T cells and antigen presenting cells. The investigators of project 3 have found that the pro-apoptotic protein BAD is crucial to the decision of activated T cells to live or die. The structure and function of BAD in different types of T cells will therefore be investigated together with the structure of BAD bound to its anti- apoptotic ligand, Bcl-xl. These three projects are coordinated not only in their common interest in lymphocyte activation but also by their common use of two technologies, which will be supported by the two Cores, in flow cytometry and digital microscopy.

淋巴细胞活化和免疫耐受一直是一个具有重要理论和实践意义的课题。该计划中的项目通过对该主题的共同兴趣联系在一起。总的来说，他们的目标是找出环境如何控制反应，或缺乏反应，或淋巴细胞对抗原。这种控制对于我们生产有效疫苗和了解自身免疫性疾病的能力都很重要。项目1中要做的实验将涉及B细胞耐受性的新模型，其中自身抗原使B细胞无应答，其应答性可以容易地再生。在项目2中，研究者将研究胸腺细胞和耐受或选择配体之间的界面的地理学。研究人员还将研究CD 8 + T细胞和抗原呈递细胞之间的界面。项目3的研究人员发现，促凋亡蛋白BAD对活化T细胞的生存或死亡至关重要。因此，将研究不同类型T细胞中BAD的结构和功能以及与其抗凋亡配体Bcl-xl结合的BAD的结构。这三个项目不仅在淋巴细胞活化方面有共同的兴趣，而且还通过共同使用两种技术进行协调，这两种技术将得到两个核心的支持，即流式细胞术和数字显微镜。