CORE--CELLULAR NEUROSCIENCE FACILITY

核心——细胞神经科学设施

• 批准号: 6652277
• 负责人: Gabriel Corfas
• 金额: $ 8.62万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652277
• 关键词: animal tissue; biomedical facility; confocal scanning microscopy; histopathology; human tissue; immunocytochemistry; in situ hybridization; mental retardation; neurosciences; tissue /cell preparation

DESCRIPTION: The Cellular Neuroscience Core was formed from the previous Histology and Electron Microscopy Cores. The Histology Core originated under the leadership of Dr. Richard Sidman, with support from the Chief Technologist, Mr. Pieter Dikkes. The Electron Microscopy Core was initiated under the leadership of Dr. Hannah Kinney, who was the first Director of the combined Cellular Neuroscience core. The current director, Dr. Gabriel Corfas, has de-emphasized the electron microscopy aspect of the service in favor of providing more extensive, high throughput services that better meet the current needs of the 37 investigators routinely using this Core. These methods of tissue preparation for structural and molecular anatomical examination include preparation of tissues for standard histology, electron microscopy, confocal microscopy, in situ hybridization, and immunocytochemistry. In addition, this Core provides perfusion of rodents for tissue examination. An emphasis of the Core is to train Center investigators and their associates in methods of tissue preparation. The Cellular Neuroscience Core also provides morphological and neuropathological consultation to investigators. Staff includes the Chief Technologist with 35 years of experience, and a to-be-named technologist with less experience.

描述：细胞神经科学核心是由以前的 组织学和电子显微镜取芯。组织学的核心起源于 理查德·西德曼博士的领导，在酋长的支持下 技术专家皮特·迪克斯先生。电子显微镜核心启动 在汉娜·金尼博士的领导下，汉娜·金尼博士是世界卫生组织第一任主任 联合细胞神经科学核心。 现任主任加布里埃尔·科法斯博士已经淡化了电子 显微镜方面的服务有利于提供更广泛、更高 更好地满足37名调查员当前需求的吞吐量服务 例行公事地使用这个核心。这些组织制备方法用于组织结构 分子解剖学检查包括为 标准组织学、电子显微镜、共聚焦显微镜、原位 杂交和免疫细胞化学。此外，该核心还提供 用于组织检查的啮齿类动物的灌流。 核心的一个重点是培训中心调查人员及其同事 在组织制备方法中。细胞神经科学核心也 为研究人员提供形态和神经病理学咨询。 员工包括拥有35年经验的首席技术专家，以及 --经验较少的技术专家。