Afferent regulation of mitral cell phenotype

二尖瓣细胞团球化的发育调控及 将通过整合解剖学、生理学和生物学特性， 和分子生物学方法。第一个目标是确定时间 双稳态和LLD这两个重要的综合体的发展历程 成熟的二尖瓣细胞树突的特性。钾离子通道介导 将鉴定双稳性并记录其发育表达。一 相关目标是确定电压门控K通道和GluR受体 基因的表达与肾小球形成、双稳态 和LLD。第二个目的是测试LLD、双稳态和 肾小球化受传入气味诱发的活动调节。完成 这是因为基因敲除小鼠在其气味传导级联中缺乏元素， 结果，缺乏模式化肾小球活动，将被检查， 用刺激剥夺小鼠，预测肾小球化的破坏， 延迟或阻止综合特性的发展。第三个目标是 评估单个未成熟根尖细胞的功能激活 树突可能在“捕获”一个，以排除其他人在 肾小球化过程。这项工作将使用焦点，激光释放笼 谷氨酸用于培养物中树突的局灶性活化。相关实验 将检查一个树突的激活是否会导致未激活的 这些细胞变得对刺激不敏感，从而导致它们的消除。 第四个目的是检验单个二尖瓣细胞的肾小球形成 树突受活性诱导的基因表达的调节。分析 将进行BMP分泌的大分子的表达， 是否以活动依赖的方式释放， 嗅觉神经输入的激活。这些分子的可能功能 将在分子阻断实验中评估成球过程。

Developmental regulation of mitral cell glomerulization and integrative properties will be addressed by integrated anatomical, physiological and molecular biological approaches. The first aim is to determine the time course of the development of bistability and LLD, two important integrative properties of mature mitral cell dendrites. Potassium channels that mediate bistability will be identified and their developmental expression documented. A related goal is to determine when voltage gated K channel and GluR receptor genes are expressed in relation to development of glomerularization, bistability and LLDs. The second aim is to test whether LLDs, bistability and glomerularization are regulated by afferent odor-evoked activity. To accomplish this, knockout mice lacking elements in their odor transduction cascade and, as a consequence, lacking patterned glomerular activity, will be examined, together with stimulus deprived mice, for predicted disruption of glomerularization and delayed or prevented development of integrative properties. The third aim is to evaluate the role that functional activation of a single immature apical dendrite may play in "capturing" that one to the exclusion of others in the process of glomerularization. This work will use focal, laser-released caged glutamate for focal activation of a dendrite in culture. Related experiments will examine whether activation of one dendrite results in the non-activated ones becoming refractory to stimulation leading, in turn, to their elimination. The fourth aim tests the hypothesis that glomerulization by a single mitral cell dendrites is regulated by activity induced gene expression. Analysis of expression of BMP secreted macromolecules will be performed and a determination made of whether they are released in an activity-dependent manner during activation of olfactory nerve inputs. Possible functions of these molecules in the glomerulization process will be assessed in molecular blocking experiments.