Core--anatomy facility

核心--解剖设施

• 批准号: 6614273
• 负责人: Kathleen S Montine
• 金额: $ 16.23万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6614273
• 关键词: Parkinson's disease; biomedical facility; human tissue; laboratory rat; tissue /cell preparation

Description (provided by applicant): The function of the Anatomy Core is to provide uniform high-quality, and cost-effective tissue preparation and data collection as well as expert technical assistance to all members of the Program Project in their efforts to further knowledge on the disease mechanisms of and potential therapies for Parkinson's disease (PD). Specifically, the Core will provide access to equipment and will conduct standard histochernical staining, Golgi impregnation, and immunohistochemical- (IHC) staining of rodent and human brain sections for all projects in this application. Standard histochernical and IHC will be utilized by all projects in this application, as will Golgi impregnation for assessment of dendritic spine density and structure in both rat (Projects 1-3) and human (Project 4). An important advantage of concentrating these efforts from all projects into the Core is that tissue is prepared by the same highly experienced personnel using the same methods and quality control protocols. This approach maximizes expertise, cost-effectiveness, and uniformity across the different projects, and provides easy comparison and integration of results obtained from the different projects.

描述（由申请人提供）： 解剖核心的功能是提供均匀的高质量和成本效益的组织 准备和数据收集以及计划项目的所有成员的专家技术援助，以进一步了解帕金森氏病（PD）的疾病机制和潜在疗法。具体而言，核心将提供对设备的访问权限，并将对本应用程序中所有项目的所有项目进行啮齿动物和人脑切片的啮齿动物和人脑切片的染色。该应用程序中的所有项目都将使用标准的历史化和IHC，而高尔基浸渍剂也将用于评估大鼠（项目1-3）和人类（项目4）的树突状脊柱密度和结构。将所有项目集中到核心的重要优势是，使用相同的方法和质量控制方案，由相同经验丰富的人员制备组织。这种方法最大程度地提高了不同项目的专业知识，成本效益和统一性，并轻松地比较和整合了从不同项目获得的结果。