NEW TOOLS TO DEVELOP MALARIA VACCINE

开发疟疾疫苗的新工具

• 批准号: 6632236
• 负责人: Victor Nussenzweig
• 金额: $ 41.25万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6632236
• 关键词: Plasmodium berghei; antibody specificity; cellular immunity; circumsporozoite protein; drug design /synthesis /production; erythrocytes; flow cytometry; green fluorescent proteins; hybrid cells; laboratory mouse; malaria vaccines; polymerase chain reaction; protein purification; subtraction hybridization; synthetic vaccines; vaccine development; vector vaccine

DESCRIPTION (Adapted from the Applicant's Abstract): They propose to 1) Identify new antigens and epitopes from malaria liver stages (Exo-erythrocytic forms (EEF). Immunization with radiation-attenuated sporozoites leads to sterile immunity, and the effector mechanisms are antibodies, cytotoxic lymphocytes (CTL) and lymophokines. In the blood circulation, antibodies attach the incoming sporozoites. If some escape and enter hepatocytes, the developing EEF can only be destroyed by T lymphocytes or by their products. Though very desirable, the systematic identification of proteins synthesized by sporozoites and carried into the EEF, or of EEF-specific proteins, has been hindered by difficulties in obtaining purified preparations of these parasite stages. They have constructed rodent malaria parasites (Plasmodium berghei) expressing GFP, the green fluorescent protein. FAC sorting will permit the purification of the highly fluorescent sporozoites from the contaminating material in the salivary glands of mosquitoes, and separation of the EEF from the vast numbers of non-infected hepatocytes.

描述（改编自申请人的摘要）：他们提出1） 从疟疾肝期（红细胞外期）中鉴定新的抗原和表位 形式（EEF）。用辐射减毒的子孢子免疫导致 无菌免疫，效应机制是抗体，细胞毒性 淋巴细胞（CTL）和淋巴细胞因子。在血液循环中， 进入的子孢子如果一些逃逸并进入肝细胞， EEF只能被T淋巴细胞或其产物破坏。虽然很 理想的是，子孢子合成的蛋白质的系统鉴定 并携带到EEF，或EEF特异性蛋白，已被阻碍， 难以获得这些寄生虫阶段的纯化制剂。他们 构建了表达GFP的啮齿类疟原虫（伯氏疟原虫）， 绿色荧光蛋白。FAC分选将允许纯化 来自唾液中污染物质的高荧光子孢子 蚊子的腺体，以及EEF从大量的蚊子中分离出来， 未感染的肝细胞。