FUNCTIONS OF EBNA-1 IN THE STABLE REPLICATION OF EBV

EBNA-1在EBV稳定复制中的作用

• 批准号: 6653091
• 负责人: Ashok A Aiyar
• 金额: $ 14.89万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653091
• 关键词: Burkitt's lymphoma; DNA replication origin; Epstein Barr virus; Saccharomyces cerevisiae; human tissue; neoplasm /cancer genetics; plasmids; tissue /cell culture; virus antigen; virus genetics; virus protein; virus replication

I will continue ongoing studies designed to characterize the mechanism by which the Epstein-Barr nuclear antigen 1 (EBNA-1) protein of Epstein-Barr virus (EBV) facilitates the stable replication of plasmids that bear oriP, EBV's plasmid origin of replication. Together my post-doctoral mentor, Dr. Bill Sugden, and I have demonstrated that oriP-plasmids are synthesized directly by the cell, and that EBNA-1 functions post- synthetically to stabilize oriP-plasmids. Our work also demonstrates that human cells can actively eliminate extra- chromosomal DNAs. This application experimentally addresses the process by which human cells eliminate plasmids, by defining when it occurs in the cell-cycle, and understanding the mechanism by which those viruses that maintain their genomes as plasmids in human cells overcome it. It also experimentally addresses the mechanism by which oriP-plasmids are stabilized so that they can be partitioned faithfully to daughter cells, and the contributions of EBNA-1 to these processes.

我将继续正在进行的研究，旨在描述 Epstein-Barr 病毒 (EBV) 的 Epstein-Barr 核抗原 1 (EBNA-1) 蛋白促进带有 oriP（EBV 的质粒复制起点）的质粒稳定复制的机制。 我和我的博士后导师 Bill Sugden 博士一起证明了 oriP 质粒是由细胞直接合成的，并且 EBNA-1 在合成后发挥作用以稳定 oriP 质粒。 我们的工作还表明，人类细胞可以主动消除染色体外的 DNA。 该应用程序通过定义质粒在细胞周期中发生的时间，并了解那些在人类细胞中以质粒形式维持其基因组的病毒克服质粒的机制，通过实验解决了人类细胞消除质粒的过程。 它还通过实验解决了 oriP 质粒稳定的机制，以便它们可以忠实地分配给子细胞，以及 EBNA-1 对这些过程的贡献。

项目成果

The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment.

• DOI: 10.1385/1-59259-192-2:221
• 发表时间: 2000
• 期刊: Methods in molecular biology
• 作者: Ashok Aiyar

Cholesterol Supplementation During Production Increases the Infectivity of Retroviral and Lentiviral Vectors Pseudotyped with the Vesicular Stomatitis Virus Glycoprotein (VSV-G).

• DOI: 10.1016/j.bej.2008.12.004
• 发表时间: 2009-05-15
• 期刊: Biochemical engineering journal
• 影响因子: 3.9
• 作者: Chen Y;Ott CJ;Townsend K;Subbaiah P;Aiyar A;Miller WM
• 通讯作者: Miller WM