Advancing Kidney Development from Pluripotent Stem Cells Using State of the Art Imaging Technologies

利用最先进的成像技术促进多能干细胞的肾脏发育

• 批准号: 2114103
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2114103
• 关键词: Advancing; Kidney; Development; Pluripotent; Stem

In this project the student will create and analyse, at several levels, human pluripotent stem cell derived mini-kidneys made in the laboratory. We have developed protocols to generate these kidney structures which contain the different parts of the kidney including the filtering unit, the glomerulus and the different tubular elements which reclaim nutrients. They develop in a similar way to in the fetus. However so far these mini kidneys lack the normal exit route for urine, the ureter. The student will use the protocol to generate stem cell derived kidney tissue and develop this to generate the ureter. This will be a critical stepping stone towards using stem cells to make properly functioning kidneys which might replace the need for people with kidney failure to receive kidney transplants donated by other people or delay their need to receive such transplants. In order to evaluate the development of stem cell -mini kidneys the student will not only use genomics technologies but also state-of-the-art imaging techniques to see the fine details inside the mini-kidneys, and this will vastly improve the detail we can observe and novelty of the work. It will allow us to develop kidney tissue with the greatest possible similarity to that found in healthy people.

在这个项目中，学生将在多个层面上创建和分析实验室制造的人类多能干细胞衍生的迷你肾脏。我们开发了生成这些肾脏结构的方案，其中包含肾脏的不同部分，包括过滤单元、肾小球和回收营养物质的不同管状元件。它们的发育方式与胎儿相似。然而到目前为止，这些迷你肾脏缺乏正常的尿液排出途径，即输尿管。学生将使用该方案生成干细胞衍生的肾组织，并开发它来生成输尿管。这将是使用干细胞制造功能正常的肾脏的关键垫脚石，这可能会取代肾功能衰竭患者接受其他人捐赠的肾移植的需要，或推迟他们接受此类移植的需要。为了评估干细胞迷你肾脏的发育，学生不仅将使用基因组学技术，还将使用最先进的成像技术来观察迷你肾脏内部的精细细节，这将极大地提高我们可以观察到的细节和工作的新颖性。它将使我们能够发育出与健康人的肾组织尽可能相似的肾组织。