CORE--CYTOGENETICS AND GENETICS

描述(申请人提供)：细胞遗传学和 遗传学的核心是为所有人提供高质量的遗传服务 癌症研究的方方面面，并向 SACI调查人员。与实验室研究相关的遗传服务将 包括标准细胞遗传学分析以及最先进的荧光 肿瘤标本和细胞系的原位杂交研究 圣安东尼奥癌症研究所的成员。核心的目标是 提供全面的基因研究并加强正在进行的计划 合作。 机构基金最近支持收购应用成像公司 核型分析系统和蔡司Axioplan II电动显微镜。 这些升级允许更高效地生产CGH、MultiFISH 和其他物种的核型分析。 遗传资源提供以下服务；标准细胞遗传学 肿瘤标本和细胞系的分析；FISH(原位荧光 杂交)检测染色体数目和/或结构的变化 中期和间期核；FiberFISH用于高分辨率标测； 用FISH分析定位转基因和其他插入探针.比较 肿瘤细胞的基因组杂交(CGH)；多重FISH用于肿瘤的核型分析 人类染色体；用于小鼠染色体分析的光谱核型； 基因分析的技术改进和技术专长，以及 用于实施新的分子技术的设施 发展起来的。

DESCRIPTION (provided by applicant): The goal of the Cytogenetics and Genetics Core is to provide high quality genetic services related to all aspects of cancer research and to provide genetic expertise and information to SACI investigators. Genetic services related to laboratory research will include standard cytogenetic analysis as well as state-of-the-art fluorescence in situ hybridization (FISH) studies of tumor specimens and cell lines to members of the San Antonio Cancer Institute. The object of the core is to provide comprehensive genetic studies and to enhance ongoing program collaborations. Institutional funds recently supported the purchase of Applied Imaging Karyotyping Systems and a Zeiss Axioplan II Motorized Microscope. These upgrades allow more efficient production of CGH, MultiFISH and other species karyotyping. The Genetic Resource offers the following services; Standard cytogenetic analysis of tumor specimens and cell lines; FISH (Fluorescence in situ hybridization) to detect chromosomal numerical and/or structural changes from metaphase and interphase nuclei; FiberFISH for high resolution mapping; Mapping transgenes and other insert probes by FISH analysis; Comparative genomic hybridization (CGH) on tumor cells; MultiFISH for karyotyping of human chromosomes; Spectral karyotyping for analysis of mouse chromosomes; and technical improvements for genetic analysis with technical expertise and facilities for the implementation of new molecular techniques as they are developed.