CORE--CYTOGENETICS AND GENETICS

核心——细胞遗传学和遗传学

• 批准号: 6651728
• 负责人: Robin Jean Leach
• 金额: $ 25.41万
• 依托单位: CTRC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-26 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6651728
• 关键词: biomedical facility; cell line; comparative genomic hybridization; cytogenetics; fluorescent in situ hybridization; genetic mapping; human genetic material tag; karyotype; neoplasm /cancer genetics

DESCRIPTION (provided by applicant): The goal of the Cytogenetics and Genetics Core is to provide high quality genetic services related to all aspects of cancer research and to provide genetic expertise and information to SACI investigators. Genetic services related to laboratory research will include standard cytogenetic analysis as well as state-of-the-art fluorescence in situ hybridization (FISH) studies of tumor specimens and cell lines to members of the San Antonio Cancer Institute. The object of the core is to provide comprehensive genetic studies and to enhance ongoing program collaborations. Institutional funds recently supported the purchase of Applied Imaging Karyotyping Systems and a Zeiss Axioplan II Motorized Microscope. These upgrades allow more efficient production of CGH, MultiFISH and other species karyotyping. The Genetic Resource offers the following services; Standard cytogenetic analysis of tumor specimens and cell lines; FISH (Fluorescence in situ hybridization) to detect chromosomal numerical and/or structural changes from metaphase and interphase nuclei; FiberFISH for high resolution mapping; Mapping transgenes and other insert probes by FISH analysis; Comparative genomic hybridization (CGH) on tumor cells; MultiFISH for karyotyping of human chromosomes; Spectral karyotyping for analysis of mouse chromosomes; and technical improvements for genetic analysis with technical expertise and facilities for the implementation of new molecular techniques as they are developed.

描述（由申请人提供）：细胞遗传学和 基因核心是提供高品质的基因相关服务， 癌症研究的各个方面，并提供遗传专业知识和信息， SACI调查员。与实验室研究有关的遗传服务将 包括标准细胞遗传学分析以及现有技术荧光 肿瘤标本和细胞系的原位杂交（FISH）研究， 圣安东尼奥癌症研究所的成员。核心的目的是 提供全面的遗传研究，并加强正在进行的计划 合作。 机构资金最近支持购买应用成像公司 核型分析系统和Zeiss Axioplan II电动显微镜。 这些升级可以更有效地生产CGH，MultiFISH 和其他物种的核型分析。 遗传资源提供以下服务：标准细胞遗传学 肿瘤标本和细胞系的分析; FISH（原位荧光 杂交），以检测染色体数目和/或结构变化， 中期和间期核; FiberFISH用于高分辨率定位; 通过FISH分析定位转基因和其它插入探针;比较 肿瘤细胞的基因组杂交（CGH）; 人类染色体;小鼠染色体光谱核型分析; 和技术改进的遗传分析与技术专长， 新的分子技术的实施设施，因为他们是 开发