High throughput ultra sensitive DNA sequencing machines

高通量超灵敏 DNA 测序仪

• 批准号: 6688789
• 负责人: VERA B. GORFINKEL
• 金额: $ 115.83万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6688789
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biotechnology; high throughput technology; nucleic acid sequence

DESCRIPTION: (provided by applicant) The main goal of the proposed project is to bring our novel DNA sequencing instrument from the research stage via the development stage to the pilot project testing stage. Based on single photon detection, a novel ultra sensitive DNA sequencing instrument has been designed and constructed. The present objectives are two-fold: (l) to complete the instrument development and to deploy a pilot high-throughput automated DNA sequencing instruments capable of carrying out production scale sequencing with a more than 10-fold decrease in the use of sequencing reagents when compared with the state-of-the-art reagent consumption in commercialized instruments, and (2) to carry out extensive testing of the instrument in a large-scale sequencing production environment. The reagent economy comes from both using low volume reactions and increased read length. The instrument development will be carried out at the State University of New York, Stony Brook, and sequencing technology development will be carried out at Cold Spring Harbor Laboratory (CSHL).

产品说明：（申请人提供）建议项目的主要目的是将我们的新型DNA测序仪从研究阶段经开发阶段带入试验项目测试阶段。基于单光子探测技术，设计并构建了一种新型的超灵敏DNA测序仪。目前的目标有两个方面：（l）完成仪器开发工作，并部署一套高通量自动化DNA测序仪器，进行生产规模的测序工作，测序试剂的用量较市售仪器的最先进试剂用量减少十倍以上，以及（2）在大规模测序生产环境中进行仪器的广泛测试。试剂经济性来自于使用低体积反应和增加的读取长度。仪器开发将在斯托尼布鲁克的纽约州立大学进行，测序技术开发将在冷泉港实验室（CSHL）进行。