High throughput ultra sensitive DNA sequencing machines

描述：(申请人提供)建议项目的主要目标是将我们的新型DNA测序仪从研究阶段通过开发阶段带到中试项目测试阶段。设计并构建了一种基于单光子探测的新型超灵敏DNA测序仪。目前的目标有两个：(L)完成仪器开发，并部署能够进行生产规模测序的高通量自动DNA测序仪试点，与商业化仪器中最先进的试剂消耗相比，测序试剂的使用量减少10倍以上，以及(2)在大规模测序生产环境中对仪器进行广泛的测试。试剂的经济性来自于使用低体积反应和增加读取长度。仪器开发将在纽约州立大学石溪分校进行，测序技术开发将在冷泉港实验室(CSHL)进行。

DESCRIPTION: (provided by applicant) The main goal of the proposed project is to bring our novel DNA sequencing instrument from the research stage via the development stage to the pilot project testing stage. Based on single photon detection, a novel ultra sensitive DNA sequencing instrument has been designed and constructed. The present objectives are two-fold: (l) to complete the instrument development and to deploy a pilot high-throughput automated DNA sequencing instruments capable of carrying out production scale sequencing with a more than 10-fold decrease in the use of sequencing reagents when compared with the state-of-the-art reagent consumption in commercialized instruments, and (2) to carry out extensive testing of the instrument in a large-scale sequencing production environment. The reagent economy comes from both using low volume reactions and increased read length. The instrument development will be carried out at the State University of New York, Stony Brook, and sequencing technology development will be carried out at Cold Spring Harbor Laboratory (CSHL).